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|Tested species reactivity||Human, Primate|
|Published species reactivity||Rhesus monkey|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified full length native human Collagen IV.|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Dot blot (DB)||Assay dependent|
|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
MA1-22148 detects Type IV Collagen in human and non-human primate samples. This antibody will not crossreact with feline, chicken, goat, rabbit, rat, or ovine.
MA1-22148 has been successfully used in ELISA, immunocytochemistry, immunofluorescence, immunohistochemistry (frozen & paraffin), dot blot and Western blot procedures. Positive control of human tissue.
The MA1-22148 immunogen is purified full length native human Collagen IV. This antibody detects an epitope on the alpha-1 and/or alpha-2 chains of human Collagen IV.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Decellularized rhesus monkey kidney as a three-dimensional scaffold for renal tissue engineering.
MA1-22148 was used in immunohistochemistry to study the use of decellularized rhesus monkey kidney as a three-dimensional scaffold in renal tissue engineering
|Nakayama KH,Batchelder CA,Lee CI,Tarantal AF||Tissue engineering. Part A (16:2207)||2010|
arresten; COL4A1 NC1 domain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen, type IV, alpha 1
BSVD; COL4A1; RATOR