|Dot blot (DB)||Assay dependent|
|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||Assay dependent|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Non-human primate|
|Published species reactivity||Rhesus monkey|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified full length native human Collagen IV.|
|Contains||15mM sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-22148 detects Type IV Collagen in human and non-human primate samples. This antibody will not crossreact with feline, chicken, goat, rabbit, rat, or ovine.
MA1-22148 has been successfully used in ELISA, immunocytochemistry, immunofluorescence, immunohistochemistry (frozen and paraffin), dot blot and Western blot procedures. Positive control of human tissue.
The MA1-22148 immunogen is purified full length native human Collagen IV. This antibody detects an epitope on the alpha-1 and/or alpha-2 chains of human Collagen IV.
Store at 4°C short term. For extended storage, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: arresten; COL4A1 NC1 domain; Collagen alpha-1(IV) chain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen, type IV, alpha 1
Gene Aliases: BSVD; COL4A1; RATOR
UniProt ID: (Human) P02462
Entrez Gene ID: (Human) 1282
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.