Invitrogen

Collagen IV Polyclonal Antibody

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Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Collagen IV in paraffin-embedded human lung cancer tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-95188) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

Collagen IV Antibody in Immunohistochemistry (IHC) — Immunofluorescence analysis of Collagen IV in human colon cancer tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-95188) at a 1 µg/mL dilution, followed by 488 conjugated goat anti-rabbit IgG using a 1:100 dilution and DAPI.

Collagen IV Antibody in Western Blot (WB) — Western blot analysis of Collagen IV in Lane 1: recombinant human Collagen protein (0.5 ng). Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-95188) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Product Details

PA5-95188

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-0.5 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	0.5-1 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669).
Conjugate	Unconjugated
Form	Lyophilized
Concentration	500 µg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 5mg BSA
Contains	0.05mg sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2806993

Product Specific Information

Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Arresten; Canstatin; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Goodpasture antigen; Tumstatin; Type IV Collagen

Gene Aliases: BSVD; CA44; COL4A1; COL4A2; COL4A3; COL4A4; ICH; POREN2; RATOR

UniProt ID: (Human) P02462, (Human) P08572, (Human) Q01955, (Human) P53420

Entrez Gene ID: (Human) 1282, (Human) 1284, (Human) 1285, (Human) 1286

Molecular Function: extracellular matrix structural protein extracellular matrix protein