Invitrogen

Collagen IV Polyclonal Antibody

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Collagen IV Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Collagen IV in human lung cancer tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-95188) at a 1 µg/mL dilution, followed by 488 conjugated Avidin using a dilution and DAPI.

Collagen IV Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Collagen IV in paraffin-embedded human lung cancer tissues. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-95188) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex and DAB.

Collagen IV Antibody in Western Blot (WB) — Western blot analysis of Collagen IV in Lane 1: recombinant human Collagen protein (0.5 ng). Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with Collagen IV polyclonal antibody (Product # PA5-95188) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

0.5-1 µg/mL

Immunofluorescence (IF)

0.5-1 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Western Blot (WB)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669).

Conjugate

Unconjugated

Form

Lyophilized

Purification

Affinity chromatography

Storage buffer

0.2mg sodium phosphate with 5mg BSA, 0.9mg NaCl

Contains

0.05mg sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 ug/mL.

Target Information

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Arresten; COL3A1; COL4A1 NC1 domain; Collagen alpha-1(IV) chain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen, type IV, alpha 1

Gene Aliases: BSVD; COL4A1; RATOR

UniProt ID: (Human) P02462

Entrez Gene ID: (Human) 1282

Function(s)

patterning of blood vessels brain development neuromuscular junction development extracellular matrix organization collagen catabolic process epithelial cell differentiation blood vessel morphogenesis retinal blood vessel morphogenesis renal tubule morphogenesis cellular response to amino acid stimulus basement membrane organization

Process(es)

extracellular matrix structural constituent protein binding extracellular matrix constituent conferring elasticity platelet-derived growth factor binding

Collagen IV Polyclonal Antibody

Product Details

Product Specific Information

Target Information

Bioinformatics

Suggested Secondary Antibodies

Material safety data sheets (MSDS)

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