Description: This monoclonal antibody 1042 recognizes human collagen Type IV. Collagens are the main components of the extracellular matrix and provide the tensile strength to tissues. There are four main types of collagen (I, II, III and IV) which contribute to the connective tissue. Expression of type IV is restricted to the basal lamina. It contains two alpha-1 and one alpha-2-like chains. Collagen Type IV is associated with disorders such as Alport syndrome and Goodpasture's syndrome.
Applications Reported: This 1042 antibody has been reported for use in immunohistochemical staining of frozen (IHC-F) and formalin-fixed paraffin embedded (IHC-P) tissue sections.
Applications Tested: This 1042 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded (FFPE) tissue with low pH antigen retrieval. This can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Arresten; Canstatin; COL4A1 NC1 domain; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; Goodpasture antigen; Tumstatin; Type IV Collagen
Gene Aliases: BSVD; CA44; COL4A1; COL4A2; COL4A3; COL4A4; ICH; POREN2; RATOR