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Product Details
PA1-85324
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A mixture of human collagen 1-5 from human placenta.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.1 mg/mL
Purification
Affinity chromatography
Storage buffer
BBS, pH 7.5, with 1% mannitol, 1% dextran
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_930067
Product Specific Information
Reconstitute with 0.5 mL distilled water.
PA1-85324 detects Collagen I,II,III,IV,V in human samples. Reactivity with collagens 1 and 2 is ~70% as strong as that with 3-5. Reactivity with human fibronectin, albumin, and immunoglobulins is <0.5%.
Rabbit anti Human collagen I/II/III/IV/V antibodyrecognizes multiple collagen types and exhibits the following reactivities in ELISA with both native and denaturated human collagen types: I & III 100%, IV & V 90%, II 80%, Human fibronectin, albumin and immunoglobulins all <0.5%.
Target Information
Collagens are highly conserved throughout evolution and are characterised by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON DENATURED three dimensional epitopes. This preparation results in a native conformation of the protein. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the gene encoding collagen 1 are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AB collagen; alpha 2 type I procollagen; alpha 2(I) procollagen; alpha 2(I)-collagen; Alpha-1 type I collagen; Alpha-1 type II collagen; Alpha-2 type I collagen; alpha1 (III) collagen; alpha1(I) procollagen; Arresten; arthroophthalmopathy, progressive (Stickler syndrome); Canstatin; cartilage collagen; Chondrocalcin; COL4A1 NC1 domain; Collagen 1; Collagen 2; Collagen 3; Collagen 4; Collagen 5; collagen alpha 1 chain type I; Collagen alpha-1(I) chain; collagen alpha-1(I) chain preproprotein; Collagen alpha-1(II) chain; Collagen alpha-1(III) chain; Collagen alpha-1(IV) chain; Collagen alpha-1(V) chain; Collagen alpha-2(I) chain; Collagen alpha-2(IV) chain; Collagen alpha-2(V) chain; Collagen alpha-3(IV) chain; Collagen alpha-3(V) chain; Collagen alpha-4(IV) chain; Collagen alpha-5(IV) chain; Collagen alpha-6(IV) chain; collagen I, alpha-2 polypeptide; collagen II, alpha-1 polypeptide; collagen IV, alpha-1 polypeptide; collagen IV, alpha-3 polypeptide; Collagen IV, alpha-4 polypeptide; collagen IV, alpha-5 polypeptide; collagen IV, alpha-6 polypeptide; collagen of basement membrane, alpha-1 chain; collagen of basement membrane, alpha-4 chain; collagen of basement membrane, alpha-5 chain; collagen of basement membrane, alpha-6; collagen of skin, tendon and bone, alpha-1 chain; collagen of skin, tendon and bone, alpha-2 chain; collagen, fetal; collagen, fetal membrane, A polypeptide; collagen, type I, alpha 1; collagen, type I, alpha 2; collagen, type II, alpha 1; collagen, type III, alpha 1; collagen, type IV, alpha 1; collagen, type IV, alpha 3 (Goodpasture antigen); collagen, type IV, alpha 4; collagen, type IV, alpha 5; collagen, type IV, alpha 6; collagen, type V, alpha 1; collagen, type V, alpha 2; collagen, type V, alpha 3; dA149D17.3; dA24A23.1; dJ889N15.4 (Collagen Alpha 6(IV)); Ehlers-Danlos syndrome type IV, autosomal dominant; Goodpasture antigen; pan Collagen; pro-(alpha)3(V) collagen; pro-alpha-1 collagen type 1; Tumstatin; type I proalpha 1; type I procollagen; type I procollagen alpha 1 chain; type V preprocollagen alpha 2 chain
Gene Aliases: ANFH; AOM; ASLN; ATS; BSVD; CA44; CA54; COL11A3; COL1A1; COL1A2; COL2A1; COL3A1; COL4A1; COL4A2; COL4A3; COL4A4; COL4A5; COL4A6; COL5A1; COL5A2; COL5A3; CXDELq22.3; DELXq22.3; DFNX6; EDS4A; EDSC; ICH; OI1; OI2; OI3; OI4; POREN2; RATOR; SEDC; STL1
UniProt ID: (Human) Q15201, (Human) P08123, (Human) P02458, (Human) Q15112, (Human) P02462, (Human) P08572, (Human) Q9BQT2, (Human) P53420, (Human) P29400, (Human) Q14031, (Human) P20908, (Human) P05997, (Human) P25940
Entrez Gene ID: (Human) 1277, (Human) 1278, (Human) 1280, (Human) 1281, (Human) 1282, (Human) 1284, (Human) 1285, (Human) 1286, (Human) 1287, (Human) 1288, (Human) 1289, (Human) 1290, (Human) 50509
Molecular Function:
extracellular matrix structural protein
extracellular matrix protein
skeletal system development
blood vessel development
osteoblast differentiation
intramembranous ossification
endochondral ossification
blood coagulation
visual perception
sensory perception of sound
positive regulation of epithelial to mesenchymal transition
negative regulation of cell-substrate adhesion
protein transport
platelet activation
extracellular matrix organization
collagen fibril organization
positive regulation of cell migration
collagen catabolic process
response to corticosteroid
response to estradiol
collagen biosynthetic process
protein localization to nucleus
tooth mineralization
response to drug
response to hydrogen peroxide
response to peptide hormone
skin morphogenesis
cellular response to fibroblast growth factor stimulus
tooth eruption
positive regulation of transcription, DNA-templated
embryonic skeletal system development
regulation of immune response
leukocyte migration
response to cAMP
response to hyperoxia
face morphogenesis
bone trabecula formation
cartilage development involved in endochondral bone morphogenesis
protein heterotrimerization
cellular response to amino acid stimulus
cellular response to mechanical stimulus
cellular response to retinoic acid
cellular response to vitamin E
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
cellular response to transforming growth factor beta stimulus
positive regulation of canonical Wnt signaling pathway
cellular response to fluoride
transforming growth factor beta receptor signaling pathway
Rho protein signal transduction
regulation of blood pressure
odontogenesis
cartilage condensation
tissue homeostasis
chondrocyte differentiation
heart morphogenesis
proteoglycan metabolic process
central nervous system development
regulation of gene expression
notochord development
inner ear morphogenesis
cartilage development
palate development
limb bud formation
embryonic skeletal joint morphogenesis
otic vesicle development
cellular response to BMP stimulus
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
cell-matrix adhesion
integrin-mediated signaling pathway
heart development
aging
response to radiation
response to mechanical stimulus
peptide cross-linking
cerebral cortex development
response to cytokine
positive regulation of Rho protein signal transduction
wound healing
extracellular fibril organization
skin development
digestive tract development
negative regulation of immune response
aorta smooth muscle tissue morphogenesis
negative regulation of neuron migration
patterning of blood vessels
brain development
neuromuscular junction development
epithelial cell differentiation
blood vessel morphogenesis
retinal blood vessel morphogenesis
renal tubule morphogenesis
basement membrane organization
activation of cysteine-type endopeptidase activity involved in apoptotic process
cell adhesion
cell surface receptor signaling pathway
blood circulation
cell proliferation
negative regulation of cell proliferation
negative regulation of endopeptidase activity
negative regulation of angiogenesis
glomerular basement membrane development
endothelial cell apoptotic process
cell migration
wound healing, spreading of epidermal cells
tendon development
integrin biosynthetic process
eye morphogenesis
regulation of cellular component organization
negative regulation of endodermal cell differentiation
ossification
extracellular matrix structural constituent
protein binding
identical protein binding
metal ion binding
platelet-derived growth factor binding
protein binding, bridging
SMAD binding
extracellular matrix structural constituent conferring tensile strength
integrin binding
extracellular matrix constituent conferring elasticity
structural molecule activity
metalloendopeptidase inhibitor activity
heparin binding
proteoglycan binding
molecular_function
collagen binding
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