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COL4A2 Antibody (PA5-104252) in ICC/IF
Immunofluorescent analysis of Collagen Pan in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with Collagen Pan polyclonal antibody (Product # PA5-104252) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Product Details
PA5-104252
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human Collagen IV alpha 2, corresponding to a region within N-terminal amino acids.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
RRID
AB_2853568
Target Information
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha-2 collagen type IV; Canstatin; Collagen alpha-2(IV) chain; procollagen, type IV, alpha 2
Gene Aliases: Col4a-2; COL4A2; ICH; POREN2
UniProt ID: (Human) P08572, (Mouse) P08122
Entrez Gene ID: (Human) 1284, (Rat) 306628, (Mouse) 12827
Molecular Function:
extracellular matrix structural protein
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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