|Tested species reactivity||Chicken|
|Published species reactivity||Rabbit|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified chicken type X collagen|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||2-4 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
MA5-14268 targets Collagen Type X in IF and IHC (P, F) applications and shows reactivity with Chicken samples.
The MA5-14268 immunogen is purified chicken type X collagen.
Type X collagen is a non-fibrillar, short-chain, network-forming collagen found in fetal hypertrophic cartilage in the growth zones of long bones, vertebrae, and ribs. It is a short collagen with a triple helical core half the length of the fibril-forming collagen type I, II, and III and a globular domain at the carboxyl end (NC-1 domain) through which the molecules assemble into an hexagonal meshwork. The function of collagen X, a unique homotrimer synthesised by hypertrophic chondrocytes, is not known but its localization and transient expression at sites of calcification suggest that it is likely to be associated with events in the early stages of endochondral bone formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of crystallization of polyhydroxyalkanoate blend on surface physicochemical properties and interactions with rabbit articular cartilage chondrocytes.
MA5-14268 was used in immunocytochemistry to study the physicochemical and biological properties of polyhydroxyalkanoate polymers with potential use in tissue bioengineering
|Zheng Z,Bei FF,Tian HL,Chen GQ||Biomaterials (26:3537)||2005|