Flow cytometric analysis of C57BL/6 mouse thymocytes incubated with rabbit anti-mouse T cells and then incubated with fresh human serum were stained using a Complement C3b monoclonal antibody (Product # MA1-70054) (filled histogram) or mouse IgG1 isotype control (open histogram).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||C57BL/6 thymocytes incubated with a rat IgG2b anti-murine Thy-1 (RmT1) and C57BL/6 serum (as a source for C1q).|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1 ug/1 million cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-70054 detects C3/C3b/iC3b in human and mouse samples.
MA1-70054 has been successfully used in flow cytometry and ELISA procedures.
The MA1-70054 immunogen is C57BL/6 thymocytes incubated with a rat IgG2b anti-mouse Thy-1 (RmT1) and C57BL/6 serum (as a source for C1q).
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptible to bacterial infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.