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Flow cytometric analysis of C57BL/6 mouse thymocytes incubated with rabbit anti-mouse T cells and then incubated with fresh human serum were stained using a Complement C3b monoclonal antibody (Product # MA1-70053) (filled histogram) or mouse IgG1 isotype control (open histogram).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||1 ug/1 million cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
MA1-70053 detects C3/C3b/iC3b in human and mouse samples.
MA1-70053 has been successfully used in flow cytometry and ELISA procedures.
The MA1-70053 immunogen is human C3.
Store at 4°C. For long term storage, aliquot and freeze unused portion at -20°C in volumes appropriat for single usage. Avoid freeze/thaw cycles.
C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins.
MA1-70053 was used in flow cytometry to study the efficacy of the serine protease C1s inhibitor TNT003 in the treatment of cold agglutinin disease
|Shi J,Rose EL,Singh A,Hussain S,Stagliano NE,Parry GC,Panicker S||Blood (123:4015)||2014|
acylation-stimulating protein cleavage product; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3a anaphylatoxin; C3b; Complement component C3; complement component C3a; complement component C3b; epididymis secretory sperm binding protein Li 62p; iC3b; prepro-C3
AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp