|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Murine complement factor C4.|
|Storage buffer||PBS with 0.1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-40047 detects C4 from mouse samples.
MA1-40047 has been successfully used in immunohistochemistry (frozen), immunoprecipitation, ELISA, immunofluorescence and Western blot applications.
The MA1-40047 immunogen is mouse complement factor C4.
Murine complement factor C4, formerly known as Gg protein, consists of alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lectin activation pathway coverage at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a, and C4b. The latter can be cleaved to result in C4c and C4d in which step all functional sites are lost. The monoclonal antibody 16D2 reacts with intact C4, C4b, and C4d. C4 is an acute phase protein that is produced by hepatocytes, monocytes, and intestinal epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.