|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||N-terminus of C5.|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Functional Assay (FN)||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-40159 detects C5/C5a from human samples.
MA1-40159 has been successfully used in ELISA, functional assays, immunoprecipitation, and Western blot applications.
The MA1-40159 immunogen is the N-terminus of C5.
This gene encodes the basic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. In addition, this gene exists as a long form and a short form due to the presence or absence of a 6.4 kb endogenous HERV-K retrovirus in intron 9.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.