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Western blot analysis of Connexin26 (Cx26) was performed by loading 30 ug of protein from mouse liver homogenate (not boiled) and 7 ul of EZ-RUN Prestained Rec Protein Ladder (Product # BP3603-500) per well onto a 10% Tris-Hcl polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked in blocking buffer composed of 20 mM Tris-HCl pH 8, 150 mM NaCl and 0.2% Tween-20 (TBST) supplemented with 5% skim milk powder (Carnation) for 1 hr at room temperature. Cx26 was detected at ~26 kDa using an anti-Cx26 antibody (Product # 13-8100, lane 1) at a dilution of 2 ug/ml in TBST supplemented with 1% skim milk powder and incubated overnight at 4°C on a rocking platform, followed by washing and incubation with a goat anti-mouse IgG horse radish peroxidase (HRP) conjugated secondary antibody at a dilution of 1:5,000 in TBST for 1 hr at room temperature. Lane 2: secondary antibody alone (primary omission). The upper band detected represents mouse IgG contamination in the mouse liver sample. Chemiluminescent detection was performed using Pierce ECL Western blotting substrate (Product # 32209). Data courtesy of Dr. James Nagy's lab.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 6 publications below|
|Western Blot (WB)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 3 publications below|
|Immunofluorescence (IF)||See 2 publications below|
13-8100 was used in the western blot analysis to successfully detect Connexin 26 in mouse liver homogenate.
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Reduced expression of Connexin26 and its DNA promoter hypermethylation in the inner ear of mimetic aging rats induced by d-galactose.
13-8100 was used in immunohistochemistry to examine the relationship between Cx26 and presbycusis
|Wu X,Wang Y,Sun Y,Chen S,Zhang S,Shen L,Huang X,Lin X,Kong W||Biochemical and biophysical research communications (452:340)||2014|
The Clouston syndrome mutation connexin30 A88V leads to hyperproliferation of sebaceous glands and hearing impairments in mice.
13-8100 was used in immunohistochemistry and western blot to investigate the effect of the Clouston syndrome mutation connexin30 A88V on the proliferation of sebaceous glands and hearing in mice
|Bosen F,Schütz M,Beinhauer A,Strenzke N,Franz T,Willecke K||FEBS letters (588:1795)||2014|
|Not Cited||Neuronal connexin expression in the cochlear nucleus of big brown bats.||Horowitz SS,Stamper SA,Simmons JA||Brain research (1197:76)||2008|
Connexin immunoreactivity in glial cells of the rat retina.
13-8100 was used in immunohistochemistry to study connexins in retinal glial cells.
|Zahs KR,Kofuji P,Meier C,Dermietzel R||The Journal of comparative neurology (455:531)||2003|
|Rat||Not Cited||Localization of connexin26 and connexin32 in putative CO(2)-chemosensitive brainstem regions in rat.||Solomon IC,Halat TJ,El-Maghrabi MR,O'Neal MH||Respiration physiology (129:101)||2001|
trans-dominant inhibition of connexin-43 by mutant connexin-26: implications for dominant connexin disorders affecting epidermal differentiation.
13-8100 was used in immunohistochemistry to investigate the effects of mutations to connexin-26 on hearing impairment.
|Rouan F,White TW,Brown N,Taylor AM,Lucke TW,Paul DL,Munro CS,Uitto J,Hodgins MB,Richard G||Journal of cell science (114:2105)||2001|
Reduced connexin 43 in eutopic endometrium and cultured endometrial stromal cells from subjects with endometriosis.
13-8100 was used in immunohistochemistry - paraffin section and western blot to investigate the expression of connexin 43 in eutopic endometrium and cultured endometrial stromal cells
|Yu J,Boicea A,Barrett KL,James CO,Bagchi IC,Bagchi MK,Nezhat C,Sidell N,Taylor RN||Molecular human reproduction (20:260)||2014|
|Human||Not Cited||Genetic ablation of the fatty acid-binding protein FABP5 suppresses HER2-induced mammary tumorigenesis.||Levi L,Lobo G,Doud MK,von Lintig J,Seachrist D,Tochtrop GP,Noy N||Cancer research (73:4770)||2013|
Tanshinone IIA increases the bystander effect of herpes simplex virus thymidine kinase/ganciclovir gene therapy via enhanced gap junctional intercellular communication.
13-8100 was used in western blot to study the role of improved intercellular gap junction communication in the mechanism by which tanshinone IIA enhances the bystander effect during HSV-thymidine kinase/ganciclovire gene therapy.
|Xiao J,Zhang G,Qiu P,Liu X,Wu Y,Du B,Li J,Zhou J,Li J,Tan Y||PloS one (8:null)||2013|
|Role of gap junctions in fluid secretion of lacrimal glands.||Walcott B,Moore LC,Birzgalis A,Claros N,Valiunas V,Ott T,Willecke K,Brink PR||American journal of physiology. Cell physiology (282:C501)||2002|
Expression of gap junction proteins connexins 26, 30, and 43 in Dupuytren's disease.
13-8100 was used in immunohistochemistry - frozen section to investigate the expression of gap junction proteins in Dupuytren disease
|Holzer LA,Cör A,Holzer G||Acta orthopaedica (85:97)||2014|
Role of p63 and the Notch pathway in cochlea development and sensorineural deafness.
13-8100 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to elucidate the role of p63 in deafness.
|Terrinoni A,Serra V,Bruno E,Strasser A,Valente E,Flores ER,van Bokhoven H,Lu X,Knight RA,Melino G||Proceedings of the National Academy of Sciences of the United States of America (110:7300)||2013|
Connexin32 can restore hearing in connexin26 deficient mice.
13-8100 was used in immunocytochemistry to study connexin 26 and connexin 32 isoforms in the cochlea.
|Degen J,Schütz M,Dicke N,Strenzke N,Jokwitz M,Moser T,Willecke K||European journal of cell biology (90:817)||2011|
|Human||Not Cited||Clinical significance of the expression of connexin26 in colorectal cancer.||Nomura S,Maeda K,Noda E,Inoue T,Fukunaga S,Nagahara H,Hirakawa K||Journal of experimental & clinical cancer research : CR (29:null)||2010|
|Human||Not Cited||Aberrant expression of connexin 26 is associated with lung metastasis of colorectal cancer.||Ezumi K,Yamamoto H,Murata K,Higashiyama M,Damdinsuren B,Nakamura Y,Kyo N,Okami J,Ngan CY,Takemasa I,Ikeda M,Sekimoto M,Matsuura N,Nojima H,Monden M||Clinical cancer research : an official journal of the American Association for Cancer Research (14:677)||2008|
|Rat||Not Cited||Role of gap junctions in spontaneous activity of the rat bladder.||Ikeda Y,Fry C,Hayashi F,Stolz D,Griffiths D,Kanai A||American journal of physiology. Renal physiology (293:F1018)||2007|
|Mouse||Not Cited||A murine living skin equivalent amenable to live-cell imaging: analysis of the roles of connexins in the epidermis.||Kandyba EE,Hodgins MB,Martin PE||The Journal of investigative dermatology (128:1039)||2008|
|Human||Not Cited||The relationship between connexins, gap junctions, tissue architecture and tumour invasion, as studied in a novel in vitro model of HPV-16-associated cervical cancer progression.||Aasen T,Hodgins MB,Edward M,Graham SV||Oncogene (22:7969)||2003|
26kD (connexin 26); 26kDa (connexin 26); beta 2; Connexin 26; connexin-26; CX26; DFNA3; DFNB1; gap junction beta-2 protein; gap junction channel protein connexin 26; gap junction membrane channel protein beta 2; gap junction protein; gap junction protein beta 2; gap junction protein, beta 2, 26kDa; GJB2; HID; KID; mutant gap junction protein beta 2; NSRD1; PPK
AI325222; Cnx26; CX26; CXN-26; DFNA3; DFNA3A; DFNB1; DFNB1A; Gjb-2; GJB2; HID; KID; NSRD1; PPK