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Immunofluorescence analysis of connexin 26 was performed on sections of adult mouse liver. Tissue sections on slides were probed for 24 h at 4°C in a humidified chamber with a mouse monoclonal anti-Cx26 (Cat. No. 33-5800), at an antibody concentration of 1-2 µg/ml diluted in 50 mM Tris-HCl, pH 7.4, containing 1.5% NaCl, 0.3% Triton X-100 (TBST) and 4% normal goat serum. After overnight incubation, sections were washed extensively for 1 h in TBST, and detection of primary antibody was performed for 1.5 h at room temperature with AlexaFluor-488-conjugated donkey anti-mouse diluted 1:600 in TBST. Sections were then was in TBST, then in TBS (without triton) and then coversliped with anti-fade medium. Images were taken on a Zeiss Z2 scanning microscope at x40 objective magnification, and show immunofluorescence labelling of Cx26 localized at gap junctions between liver hepatocytes. Data courtesy of Dr. James Nagy's lab.
|Tested species reactivity||Rat , Mouse|
|Published species reactivity||Rat , Mouse , Human , Chicken , Guinea pig|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||A 13 amino acid synthetic peptide derived from the C-terminus of the mouse Connexin 26 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
33-5800 was used in the immunofluorescence and western blot analysis to successfully detect Connexin26 in mouse liver sections and homogenate, respectively.
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Connexin26 (GJB2) deficiency reduces active cochlear amplification leading to late-onset hearing loss.
33-5800 was used in immunohistochemistry to study hearing loss using a time-controlled, inducible gene knockout technique to knockout Cx26 expression in the cochlea
|Zhu Y,Chen J,Liang C,Zong L,Chen J,Jones RO,Zhao HB||Neuroscience (284:719)||2015|
Correlations of differentially expressed gap junction connexins Cx26, Cx30, Cx32, Cx43 and Cx46 with breast cancer progression and prognosis.
33-5800 was used in immunohistochemistry to correlate connexin expression with tumor progression and prognosis in primary breast cancers
|Teleki I,Szasz AM,Maros ME,Gyorffy B,Kulka J,Meggyeshazi N,Kiszner G,Balla P,Samu A,Krenacs T||PloS one (9:null)||2014|
Active cochlear amplification is dependent on supporting cell gap junctions.
33-5800 was used in immunohistochemistry to show that gap junctions between cochlear supporting cells have a critical role in active cochlear amplification in vivo.
|Zhu Y,Liang C,Chen J,Zong L,Chen GD,Zhao HB||Nature communications (4:null)||2013|
Localization of connexin26 and connexin32 in putative CO(2)-chemosensitive brainstem regions in rat.
||Solomon IC,Halat TJ,El-Maghrabi MR,O'Neal MH||Respiration physiology (129:101)||2001|
trans-dominant inhibition of connexin-43 by mutant connexin-26: implications for dominant connexin disorders affecting epidermal differentiation.
33-5800 was used in immunohistochemistry to investigate the effects of mutations to connexin-26 on hearing impairment.
|Rouan F,White TW,Brown N,Taylor AM,Lucke TW,Paul DL,Munro CS,Uitto J,Hodgins MB,Richard G||Journal of cell science (114:2105)||2001|
Gap junctional coupling is essential for epithelial repair in the avian cochlea.
33-5800 was used in immunocytochemistry to study the role of gap junctional coupling in epithelial repair in the avian cochlea
|Jagger DJ,Nickel R,Forge A||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:15851)||2014|
Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.
33-5800 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus
|Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI||The European journal of neuroscience (39:757)||2014|
Deafness induced by Connexin 26 (GJB2) deficiency is not determined by endocochlear potential (EP) reduction but is associated with cochlear developmental disorders.
33-5800 was used in immunohistochemistry - frozen section to study the mechanism of deafness induced by Connexin 26 deficiency
|Chen J,Chen J,Zhu Y,Liang C,Zhao HB||Biochemical and biophysical research communications (448:28)||2014|
Connexin30-mediated intercellular communication plays an essential role in epithelial repair in the cochlea.
33-5800 was used in immunohistochemistry - frozen section to determine the role of connexin 30 in epithelial repair following injury in the organ of Corti.
|Forge A,Jagger DJ,Kelly JJ,Taylor RR||Journal of cell science (126:1703)||2013|
Prostaglandin-induced cervical remodelling in humans in the first trimester is associated with increased expression of specific tight junction, but not gap junction proteins.
33-5800 was used in immunohistochemistry - frozen section and western blot to assess the role of tight junctions and gap proteins in cervical remodeling.
|Ghulé VV,Gray C,Galimberti A,Anumba DO||Journal of translational medicine (10:null)||2012|
Targeted epidermal expression of mutant Connexin 26(D66H) mimics true Vohwinkel syndrome and provides a model for the pathogenesis of dominant connexin disorders.
33-5800 was used in immunohistochemistry - frozen section to study the role of connexin 26 in dominantly inherited skin disease.
|Bakirtzis G,Choudhry R,Aasen T,Shore L,Brown K,Bryson S,Forrow S,Tetley L,Finbow M,Greenhalgh D,Hodgins M||Human molecular genetics (12:1737)||2003|
Missense mutations in GJB2 encoding connexin-26 cause the ectodermal dysplasia keratitis-ichthyosis-deafness syndrome.
33-5800 was used in immunohistochemistry - frozen section to investigate the role of heterozygous missense mutations in the connexin-26 gene in keratitis-ichthyosis-deafness syndrome.
|Richard G,Rouan F,Willoughby CE,Brown N,Chung P,Ryynänen M,Jabs EW,Bale SJ,DiGiovanna JJ,Uitto J,Russell L||American journal of human genetics (70:1341)||2002|
p63 attenuates epithelial to mesenchymal potential in an experimental prostate cell model.
33-5800 was used in western blot to study the role of p63 in epithelial homeostasis and development
|Olsen JR,Oyan AM,Rostad K,Hellem MR,Liu J,Li L,Micklem DR,Haugen H,Lorens JB,Rotter V,Ke XS,Lin B,Kalland KH||PloS one (8:null)||2013|
Connexin26 expression in brain parenchymal cells demonstrated by targeted connexin ablation in transgenic mice.
33-5800 was used in western blot to study connexin 26 in astrocytes.
|Nagy JI,Lynn BD,Tress O,Willecke K,Rash JE||The European journal of neuroscience (34:263)||2011|
Functional heterotypic interactions between astrocyte and oligodendrocyte connexins.
||Magnotti LM,Goodenough DA,Paul DL||Glia (59:26)||2011|
|Guinea pig||Not Cited||
Distinct and gradient distributions of connexin26 and connexin30 in the cochlear sensory epithelium of guinea pigs.
||Zhao HB,Yu N||The Journal of comparative neurology (499:506)||2006|
In vivo and in vitro expression of connexins in the human corneal epithelium.
||Shurman DL,Glazewski L,Gumpert A,Zieske JD,Richard G||Investigative ophthalmology & visual science (46:1957)||2005|
The relationship between connexins, gap junctions, tissue architecture and tumour invasion, as studied in a novel in vitro model of HPV-16-associated cervical cancer progression.
||Aasen T,Hodgins MB,Edward M,Graham SV||Oncogene (22:7969)||2003|
Gjb-2, CXN-26, Cnx26, AI325222, Cx26
connexin 26, connexin-26, gap junction beta-2 protein, gap junction channel protein connexin 26, gap junction membrane channel protein beta 2, GJB2, Connexin 26, gap junction protein, beta 2, 26kDa (connexin 26), CX26, DFNA3, DFNB1, HID, KID, NSRD1, PPK, gap junction protein beta 2, 26kD (connexin 26)