Immunofluorescent analysis of Connexin 30 was performed on paraffin-embedded mouse thalamus tissue sections. To expose target proteins, endogenous peroxide blocking followed by heat induced epitope retrieval was performed using Tris-EDTA (pH 9. 0) buffer for 15 minutes. Following antigen retrieval, tissues were blocked in 0. 2% BSA with 0. 1% cold fish skin gelatin in 1X PBS for 1 hour in a humidified chamber. The tissues were then probed with a Connexin 30 ABfinity™ recombinant rabbit monoclonal antibody (Product # 700258) at a dilution of 0. 5ug/ml for 3 hours at room temperature in a humidified chamber. Detection was performed using an HRP-conjugated goat anti-rabbit secondary antibody (Product # G21234), followed by colorimetric detection using DAB. Note staining of the lateral geniculate nucleus (left) and part of the ventral lateral nucleus (right).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 241-261 of P70689.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with human, rat, hamster and bovine based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Gap junctions are responsible for transport of ions and metabolites between adjacent cells. These structures are made of two hemichannels, each formed by six connexin molecules. Connexin 30, coded by Cjb6 gene, is a member of the connexin family and is highly expressed in brain and skin. Immunohistochemically connexin 30 was localized in astrocytes, at gap junctions between these cells and on the astrocyte side of gap junctions between astrocytes and oligodendrocytes. Co-localization with connexin 43 was also observed in this study. Cytoskeleton, especially actin filaments, are important components in the processes of assembly, trafficking and stabilization of connexin 30 gap junctions. Knock-out mice studies have also demonstrated that connexin 30 deficiency impairs renal tubular ATP release and pressure natriuresis highlighting the importance of this molecule in kidney. Further, in vitro studies have also demonstrated that connexin 30 overexpression enhances cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Connexins, E-cadherin, Claudin-7 and ß-catenin transiently form junctional nexuses during the post-natal mammary gland development.
700258 was used in immunoprecipitation to assess the transiently formed junctional nexuses during post-natal mammary gland development by E-cadherins, connexins, beta-catenin, and claudin-7
|Dianati E,Poiraud J,Weber-Ouellette A,Plante I||Developmental biology (416:52)||2016|
Connexin 43 in astrocytes contributes to motor neuron toxicity in amyotrophic lateral sclerosis.
700258 was used in immunohistochemistry - frozen section and western blot to learn the contribution to motor neuron toxicity in amyotrophic lateral sclerosis via connexin 43 in astrocytes
|Almad AA,Doreswamy A,Gross SK,Richard JP,Huo Y,Haughey N,Maragakis NJ||Glia (64:1154)||2016|