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Immunofluorescence analysis of Connexin-30 was done on 70% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Connexin-30 Rabbit polyclonal Antibody (712200) at 2µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing junctional localization. Panel e shows no primary antibody control. The images were captured at 20X magnification.
|Tested species reactivity||Human , Mouse , Rat , Cat|
|Published species reactivity||Rat , Mouse , Human , Chicken , Guinea pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the unique C-terminus of the mouse Connexin 30 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Gap junctions allow the transport of ions and metabolites between the cytoplasm of adjacent cells. They are formed by two hemichannels, made up of six connexin proteins assembled in groups. Each connexin protein has four transmembrane segments, two extracellular loops, a cytoplasmic loop formed between the two inner transmembrane segments, and the N- and C-terminus both being in the cytoplasm. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form of the gap junction is present. This gene encodes one of the connexin proteins. Mutations in this gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Isoform-specific phosphorylation-dependent regulation of connexin hemichannels.
71-2200 was used in western blot to study protein kinase c-mediated regulation of connexin 30 hemichannels.
|Alstrøm JS,Hansen DB,Nielsen MS,MacAulay N||Journal of neurophysiology (114:3014)||2015|
Mammary gland specific knockdown of the physiological surge in Cx26 during lactation retains normal mammary gland development and function.
71-2200 was used in western blot to determine if patients with loss-of-function Cx26 mutations have a greater susceptibility to impaired breast development
|Stewart MK,Plante I,Bechberger JF,Naus CC,Laird DW||PloS one (9:null)||2014|
Cerebral ischemic injury is enhanced in a model of oculodentodigital dysplasia.
71-2200 was used in western blot to assess the effect of the +/G60S mutation in connexin 43 following stroke.
|Kozoriz MG,Lai S,Vega JL,Sáez JC,Sin WC,Bechberger JF,Naus CC||Neuropharmacology (75:549)||2013|
Connexin26 in adult rodent central nervous system: demonstration at astrocytic gap junctions and colocalization with connexin30 and connexin43.
71-2200 was used in western blot to assess the regional, cellular, and subcellular localization of Cx26 in mouse and rat neural tissue.
|Nagy JI,Li X,Rempel J,Stelmack G,Patel D,Staines WA,Yasumura T,Rash JE||The Journal of comparative neurology (441:302)||2001|
Connexin26 (GJB2) deficiency reduces active cochlear amplification leading to late-onset hearing loss.
71-2200 was used in immunohistochemistry to study hearing loss using a time-controlled, inducible gene knockout technique to knockout Cx26 expression in the cochlea
|Zhu Y,Chen J,Liang C,Zong L,Chen J,Jones RO,Zhao HB||Neuroscience (284:719)||2015|
The Clouston syndrome mutation connexin30 A88V leads to hyperproliferation of sebaceous glands and hearing impairments in mice.
71-2200 was used in immunohistochemistry to investigate the effect of the Clouston syndrome mutation connexin30 A88V on the proliferation of sebaceous glands and hearing in mice
|Bosen F,Schütz M,Beinhauer A,Strenzke N,Franz T,Willecke K||FEBS letters (588:1795)||2014|
Active cochlear amplification is dependent on supporting cell gap junctions.
71-2200 was used in immunohistochemistry to show that gap junctions between cochlear supporting cells have a critical role in active cochlear amplification in vivo.
|Zhu Y,Liang C,Chen J,Zong L,Chen GD,Zhao HB||Nature communications (4:null)||2013|
BAAV mediated GJB2 gene transfer restores gap junction coupling in cochlear organotypic cultures from deaf Cx26Sox10Cre mice.
71-2200 was used in immunohistochemistry to study the expression and function of connexin26 and connexin30 in the ears of transgenic mice.
|Crispino G,Di Pasquale G,Scimemi P,Rodriguez L,Galindo Ramirez F,De Siati RD,Santarelli RM,Arslan E,Bortolozzi M,Chiorini JA,Mammano F||PloS one (6:null)||2011|
Analysis of connexin subunits required for the survival of vestibular hair cells.
71-2200 was used in immunohistochemistry to test if vestibular hair cells are degraded in connexin knockout mice.
|Qu Y,Tang W,Dahlke I,Ding D,Salvi R,Söhl G,Willecke K,Chen P,Lin X||The Journal of comparative neurology (504:499)||2007|
Connexin immunoreactivity in glial cells of the rat retina.
71-2200 was used in immunohistochemistry to study connexins in retinal glial cells.
|Zahs KR,Kofuji P,Meier C,Dermietzel R||The Journal of comparative neurology (455:531)||2003|
Gap junctional coupling is essential for epithelial repair in the avian cochlea.
71-2200 was used in immunocytochemistry to study the role of gap junctional coupling in epithelial repair in the avian cochlea
|Jagger DJ,Nickel R,Forge A||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:15851)||2014|
Spatiotemporal changes in Cx30 and Cx43 expression during neuronal differentiation of P19 EC and NT2/D1 cells.
71-2200 was used in immunocytochemistry to examine the expression and roles Cx30, Cx36, and Cx43 in neuronal differentiation
|Wan CK,O'Carroll SJ,Kim SL,Green CR,Nicholson LF||Cell biology international reports (20:13)||2013|
Comparison of bidirectional and bicistronic inducible systems for coexpression of connexin genes and fluorescent reporters.
71-2200 was used in immunocytochemistry to study Cx30 and Cx43 using inducible expression systems.
|Wan CK,Shaikh SB,Green CR,Nicholson LF||Analytical biochemistry (431:90)||2012|
Connexin32 can restore hearing in connexin26 deficient mice.
71-2200 was used in immunocytochemistry to study connexin 26 and connexin 32 isoforms in the cochlea.
|Degen J,Schütz M,Dicke N,Strenzke N,Jokwitz M,Moser T,Willecke K||European journal of cell biology (90:817)||2011|
Deafness induced by Connexin 26 (GJB2) deficiency is not determined by endocochlear potential (EP) reduction but is associated with cochlear developmental disorders.
71-2200 was used in immunohistochemistry - frozen section to study the mechanism of deafness induced by Connexin 26 deficiency
|Chen J,Chen J,Zhu Y,Liang C,Zhao HB||Biochemical and biophysical research communications (448:28)||2014|
Expression of gap junction proteins connexins 26, 30, and 43 in Dupuytren's disease.
71-2200 was used in immunohistochemistry - frozen section to investigate the expression of gap junction proteins in Dupuytren disease
|Holzer LA,Cör A,Holzer G||Acta orthopaedica (85:97)||2014|
Connexin dynamics in the privileged wound healing of the buccal mucosa.
71-2200 was used in immunohistochemistry - frozen section to determine connexin changes during check wound healing.
|Davis NG,Phillips A,Becker DL||Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society (21:571)||2013|
Connexin30-mediated intercellular communication plays an essential role in epithelial repair in the cochlea.
71-2200 was used in immunohistochemistry - frozen section to determine the role of connexin 30 in epithelial repair following injury in the organ of Corti.
|Forge A,Jagger DJ,Kelly JJ,Taylor RR||Journal of cell science (126:1703)||2013|
Prostaglandin-induced cervical remodelling in humans in the first trimester is associated with increased expression of specific tight junction, but not gap junction proteins.
71-2200 was used in immunohistochemistry - frozen section and western blot to assess the role of tight junctions and gap proteins in cervical remodeling.
|Ghulé VV,Gray C,Galimberti A,Anumba DO||Journal of translational medicine (10:null)||2012|
Targeted epidermal expression of mutant Connexin 26(D66H) mimics true Vohwinkel syndrome and provides a model for the pathogenesis of dominant connexin disorders.
71-2200 was used in immunohistochemistry - frozen section to study the role of connexin 26 in dominantly inherited skin disease.
|Bakirtzis G,Choudhry R,Aasen T,Shore L,Brown K,Bryson S,Forrow S,Tetley L,Finbow M,Greenhalgh D,Hodgins M||Human molecular genetics (12:1737)||2003|
Functional heterotypic interactions between astrocyte and oligodendrocyte connexins.
||Magnotti LM,Goodenough DA,Paul DL||Glia (59:26)||2011|
|Guinea pig||Not Cited||
Distinct and gradient distributions of connexin26 and connexin30 in the cochlear sensory epithelium of guinea pigs.
||Zhao HB,Yu N||The Journal of comparative neurology (499:506)||2006|
In vivo and in vitro expression of connexins in the human corneal epithelium.
||Shurman DL,Glazewski L,Gumpert A,Zieske JD,Richard G||Investigative ophthalmology & visual science (46:1957)||2005|
CX30, ECTD2, ED2, HED, D14Bwg0506e, Cx30, EDH, DFNA3, DFNB1B, HED2, AA958971, DFNA3B
connexin 30, connexin-30, ectodermal dysplasia 2, hidrotic (Clouston syndrome), gap junction beta-6 protein, gap junction protein, beta 6 (connexin 30), gap junction membrane channel protein beta 6, CXN-30, CX30, Gjb6, Gap junction beta-6 protein