This antibody is specific for the Connexin 30. Cross-reactivity with the highly related Connexin 26 protein or with other Connexin family members has not been detected. Reactivity of this antibody has been confirmed by western blotting using cell lysates derived from adult rat, mouse, human, and cat brains.
Intracellular communication mediated by gap junctions plays an important role in a variety of cellular processes including: homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. The specificity of the gap junction is determined by which connexin proteins comprise the hemichannel. In the past, connexin protein names were based on their molecular weight, however the new nomenclature uses sequential numbers based on which form of the gap junction is present. Connexins are highly homologous proteins encoded by a multigene family composed of at least 12 distinct members. Each of the connexins exhibits similar structural features including a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail of varying length. Comparison of the amino acid sequences of the various connexin family members indicate that the two areas of greatest divergence amongst them are the intracellular loop connecting the second and third transmembrane segments and the C-terminal tail. These domains are, therefore, thought to mediate connexin-type specific properties including: phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of gap-junctional communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, postranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has be found to correlate with tumor progression and metastasis. Connexin 30 (Cx30) is a newly identified member of the connexin gene family. Cx30 was isolated by screening a genomic mouse library with a rat connexin 26 (Cx26) probe. The Cx30 protein is most closely related to the Cx26 protein (77% amino acid sequence identity). The expression patterns of Cx30 and Cx26 are clearly distinct, specifically, Cx30 is highly expressed in adult skin and brain; however, it cannot be detected in the embryonic and fetal brain. On the other hand, Cx26 is expressed highly in prenatal brain and decreases after birth. Mutations in the Cx30 gene have been found in some forms of deafness and in some families with hidrotic ectodermal dysplasia.
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Protein Aliases: connexin 30; Connexin-30; CX30; CXN-30; ectodermal dysplasia 2, hidrotic (Clouston syndrome); Gap junction beta-6 protein; gap junction membrane channel protein beta 6; gap junction protein, beta 6 (connexin 30); gap junction protein, beta 6, 30kDa; Gjb6
Gene Aliases: AA958971; CX30; Cxn-30; D14Bwg0506e; DFNA3; DFNA3B; DFNB1B; ECTD2; ED2; EDH; GJB6; HED; HED2