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Immunohistochemistry of the conduction system of mouse heart Immunolabeling in the conduction system of mouse heart where this connexin is localized to gap junctions at intercalated discs. Connexin was detected with Connexin 30.2, Rabbit Polyclonal Antibody - Unconjugated (Cat. no. 407400, 2 µg/ml) as the primary antibody and a fluorescein conjugated secondary antibody. Images provide by Dr. James Nagy, University of Manitoba.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the c-term region of the mouse connexin 30.2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
40-7400 was used in the western blot analysis to successfully detect Connexin 30.2 in HeLa cells transfected with Cx30.2 and in immunofluorescence (IHC) analysis to successfully detect Connexin 30.2 in mouse heart sections.
Gap junctions are responsible for transport of ions and metabolites between adjacent cells. These structures are made of two hemichannels, each formed by six connexin molecules. Connexin 30, coded by Cjb6 gene, is a member of the connexin family and is highly expressed in brain and skin. Immunohistochemically connexin 30 was localized in astrocytes, at gap junctions between these cells and on the astrocyte side of gap junctions between astrocytes and oligodendrocytes. Co-localization with connexin 43 was also observed in this study. Cytoskeleton, especially actin filaments, are important components in the processes of assembly, trafficking and stabilization of connexin 30 gap junctions. Knock-out mice studies have also demonstrated that connexin 30 deficiency impairs renal tubular ATP release and pressure natriuresis highlighting the importance of this molecule in kidney. Further, in vitro studies have also demonstrated that connexin 30 overexpression enhances cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
connexin 30.2; connexin 31.9; connexin-31.9; Cx31.9; gap junction alpha-11 protein; gap junction chi-1 protein; gap junction delta-3 protein; gap junction membrane channel protein chi 1; gap junction protein, delta 3, 31.9kDa; GJA11; GJC1; GJD3
Cx30.2; CX31.9; GJA11; GJC1; GJD3