Western blot analysis of Connexin 40 using A) 30 µg 293T whole cell lysate (B) 30 µg A431 whole cell lysate and C) 30 µg HepG2 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a Connexin 40 polyclonal antibody (Product # PA5-34964) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 136 and 235 of Connexin 40|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34964 targets Connexin 40 in WB applications and shows reactivity with Human samples.
The PA5-34964 immunogen is synthetic peptide corresponding to a region within amino acids 136 and 235 of Connexin 40.
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.