Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
|Tested species reactivity||Mouse|
|Published species reactivity||Rat, Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal region of the mouse, rat, Chinese hamster, and golden hamster connexin 45 (Cx45, GJA7, gap junction alpha-7) protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Direct Exposure to Ethanol Disrupts Junctional Cell-Cell Contact and Hippo-YAP Signaling in HL-1 Murine Atrial Cardiomyocytes.
40-7000 was used in western blot to investigate junctional intercellular communication, mechanotransduction through cytoskeletal organization, and the hippo-YAP pathway in cardiac damage caused by direct exposure to ethanol.
|Noritake K,Aki T,Funakoshi T,Unuma K,Uemura K||PloS one (10:null)||2015|
Connexin and AMPA receptor expression changes over time in the rat olfactory bulb.
40-7000 was used in western blot to test if circadian electrical oscillations are synchronized by changes in the expression of connexins and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits.
|Corthell JT,Fadool DA,Trombley PQ||Neuroscience (222:38)||2012|
|Human||1:200||Connexin expression and gap junctional coupling in human cumulus cells: contribution to embryo quality.||Wang HX,Tong D,El-Gehani F,Tekpetey FR,Kidder GM||Journal of cellular and molecular medicine (13:972)||2009|
Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.
40-7000 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus
|Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI||The European journal of neuroscience (39:757)||2014|
Sex differences in expression and subcellular localization of heart rhythm determinant proteins.
40-7000 was used in immunocytochemistry to investigate the effect of sex differences on cardiac gene and protein expression.
|Thomas NM,Jasmin JF,Lisanti MP,Iacobas DA||Biochemical and biophysical research communications (406:117)||2011|
Localization of neurones expressing the gap junction protein Connexin45 within the adult spinal dorsal horn: a study using Cx45-eGFP reporter mice.
40-7000 was used in immunohistochemistry to identify Cx45-positive neurones in the adult spinal dorsal horn
|Chapman RJ,Lall VK,Maxeiner S,Willecke K,Deuchars J,King AE||Brain structure & function (218:751)||2013|
||Connexin expression and gap junctional coupling in human cumulus cells: contribution to embryo quality.||Wang HX,Tong D,El-Gehani F,Tekpetey FR,Kidder GM||Journal of cellular and molecular medicine (13:972)||2009|
|Mouse||Not Cited||Ultrastructural localization of connexins (Cx36, Cx43, Cx45), glutamate receptors and aquaporin-4 in rodent olfactory mucosa, olfactory nerve and olfactory bulb.||Rash JE,Davidson KG,Kamasawa N,Yasumura T,Kamasawa M,Zhang C,Michaels R,Restrepo D,Ottersen OP,Olson CO,Nagy JI||Journal of neurocytology (34:307)||2005|
Connexin 45; connexin-45; Cx45; gap junction alpha-7 protein; gap junction gamma-1 protein; gap junction membrane channel protein alpha 7
C130009G16Rik; Cnx45; Cx45; Cxn-45; Gja-7; Gja7; Gjc1