|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 131-144 of Q64448.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5-2 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with rat and hamster based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Connexin 46 (Cx46), also known as Gap Junction Protein Alpha-3 (GJA3), CAE3, CX46 and C2P3, maps to human chromosome 13q11-q12 and encodes a 46 kDa protein. Cx46, along with Cx50, is principally expressed in the lens of the eye. Cx46 mediates intercellular interactions during development and is necessary for the survival of neural crest cells. Cx46 forms gap junctions that connect lens fiber cells and are crucial for maintaining lens transparency and normal lens function. Mutations of Cx46 result in severe cataracts of the lens. Individual knockouts of Cx46 and Cx50 lead to changes in the rate of lens fiber cell differentiation and cell size, thus the interaction of Cx46 and Cx50 is required for proper organization of fiber cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Beta-1 integrin is important for the structural maintenance and homeostasis of differentiating fiber cells.
700384 was used in immunohistochemistry - frozen section to study the effect of deletion of beta1-integrin solely from the lens fiber cells
|Scheiblin DA,Gao J,Caplan JL,Simirskii VN,Czymmek KJ,Mathias RT,Duncan MK||The international journal of biochemistry and cell biology (50:132)||2014|
Cx50 requires an intact PDZ-binding motif and ZO-1 for the formation of functional intercellular channels.
700384 was used in immunocytochemistry to investigate the interactions between the PDZ domain-binding motifs of connexins and the scaffolding protein ZO-1
|Chai Z,Goodenough DA,Paul DL||Molecular biology of the cell (22:4503)||2011|