Invitrogen

Connexin 50 Monoclonal Antibody (C6)

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Connexin 50 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Connexin 50 was done on 90% confluent log phase CaCo-2 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with Connexin 50 Mouse Monoclonal Antibody (Product # 33-4300) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product # A12381). Panel d is a merged image showing junctional localization of Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059). and panel e is a no primary antibody control. The images were captured at 20X magnification.

Connexin 50 Antibody in Western Blot (WB) — Western blot analysis of Connexin 50 was performed by loading 20 µg of Rat Brain (lane1) and Mouse Brain (lane2) lysates using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for 1 hour at room temperature. Connexin 50 was detected at ~48 kDa using Connexin 50 Mouse Monoclonal Antibody (Product # 33-4300) at 0.5-1 µg/mL in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse IgG - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Connexin 50 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Published figure using Connexin 50 monoclonal antibody (Product # 33-4300) in Western Blot and Immunohistochemistry

Product Details

33-4300

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-dependent	View 8 publications 8 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunohistochemistry (Frozen) (IHC (F))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	Assay-dependent	View 2 publications 2 publications
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Sheep, Rat
Published species	Human, Mouse, Pig, Rabbit, Rat
Host/Isotype	Mouse / IgM, kappa
Class	Monoclonal
Type	Antibody
Clone	C6
Immunogen	This antibody was prepared against urea/alkali stripped sheep lens membranes.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Size-exclusion chromatography
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533120

Target Information

This gene encodes a transmembrane connexin protein that is necessary for lens growth and maturation of lens fiber cells. The encoded protein is a component of gap junction channels and functions in a calcium and pH-dependent manner. Mutations in this gene have been associated with zonular pulverulent cataracts, nuclear progressive cataracts, and cataract-microcornea syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Connexin Signaling Is Involved in the Reactivation of a Latent Stem Cell Niche after Spinal Cord Injury.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fabbiani G,Reali C,Valentín-Kahan A,Rehermann MI,Fagetti J,Falco MV,Russo RE

33-4300 was used in Immunohistochemistry to study the role of connexin signalling in the central canal in the reactivation of latent stem cell after spinal cord injury.

Wed Mar 11 00:00:00 EDT 2020

Cholesterol metabolism and Cx43, Cx46, and Cx50 gap junction protein expression and localization in normal and diabetic and obese ob/ob and db/db mouse testes.

American journal of physiology. Endocrinology and metabolism

Pelletier RM,Akpovi CD,Chen L,Vitale ML

33-4300 was used in Western Blotting to indicate that the db mutation-induced impairment of meiosis may arise from imbalances in cholesterol metabolism and upregulated Cx43 expression and phosphorylation in tubules.

Mon Jan 01 00:00:00 EST 2018

Connexin 50 modulates Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells.

Cell and tissue research

Rodriguez-Jimenez FJ,Alastrue A,Stojkovic M,Erceg S,Moreno-Manzano V

33-4300 was used in immunocytochemistry and western blot to investigate modulation of Sox2 expression in spinal-cord-derived ependymal stem/progenitor cells by connexin 50

Mon Aug 01 00:00:00 EDT 2016

Expression and Localization of Connexins in the Outer Retina of the Mouse.

Journal of molecular neuroscience : MN

Bolte P,Herrling R,Dorgau B,Schultz K,Feigenspan A,Weiler R,Dedek K,Janssen-Bienhold U

33-4300 was used in immunohistochemistry (frozen) to investigate the counterpart of connexin36 at gap junctions between rods and cones.

Mon Feb 01 00:00:00 EST 2016

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

Published figure using Connexin 50 monoclonal antibody (Product # 33-4300) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2015

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

Published figure using Connexin 50 monoclonal antibody (Product # 33-4300) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2015

Connexin50 couples axon terminals of mouse horizontal cells by homotypic gap junctions.

The Journal of comparative neurology

Dorgau B,Herrling R,Schultz K,Greb H,Segelken J,Ströh S,Bolte P,Weiler R,Dedek K,Janssen-Bienhold U

Published figure using Connexin 50 monoclonal antibody (Product # 33-4300) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2015

Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.

Journal of cellular physiology

Shahidullah M,Mandal A,Beimgraben C,Delamere NA

Sun Apr 01 00:00:00 EDT 2012

Hyposmotic stress causes ATP release and stimulates Na,K-ATPase activity in porcine lens.

Journal of cellular physiology

Shahidullah M,Mandal A,Beimgraben C,Delamere NA

Sun Apr 01 00:00:00 EDT 2012

PKCγ, role in lens differentiation and gap junction coupling.

Current eye research

Das S,Wang H,Molina SA,Martinez-Wittinghan FJ,Jena S,Bossmann LK,Miller KA,Mathias RT,Takemoto DJ

33-4300 was used in western blot to use knock out mice to determine the role of PKCgamma in the regulation of gap junction coupling in the normal lens.

Fri Jul 01 00:00:00 EDT 2011

Expression of connexins in chromaffin cells of normal human adrenals and in benign and malignant pheochromocytomas.

Annals of the New York Academy of Sciences

Willenberg HS,Schott M,Saeger W,Tries A,Scherbaum WA,Bornstein SR

33-4300 was used in immunohistochemistry - paraffin section to examine connexin expression in chromaffin cells of normal human adrenal glands and benign and malignant pheochromocytomas

Tue Aug 01 00:00:00 EDT 2006

Gap junctional coupling and connexin immunoreactivity in rabbit retinal glia.

Visual neuroscience

Zahs KR,Ceelen PW

33-4300 was used in immunohistochemistry - frozen section to study connexins in mammalian Muller cells

Mon Jun 05 00:00:00 EDT 2006

Regulation of lens cell-to-cell communication by activation of PKCgamma and disassembly of Cx50 channels.

Investigative ophthalmology & visual science

Zampighi GA,Planells AM,Lin D,Takemoto D

Thu Sep 01 00:00:00 EDT 2005

Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma.

Molecular vision

Lin D,Lobell S,Jewell A,Takemoto DJ

Mon Sep 27 00:00:00 EDT 2004

Differential phosphorylation of connexin46 and connexin50 by H2O2 activation of protein kinase Cgamma.

Molecular vision

Lin D,Lobell S,Jewell A,Takemoto DJ

Mon Sep 27 00:00:00 EDT 2004

Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells.

Journal of cell science

Klepeis VE,Cornell-Bell A,Trinkaus-Randall V

Sat Dec 01 00:00:00 EST 2001

Growth factors but not gap junctions play a role in injury-induced Ca2+ waves in epithelial cells.

Journal of cell science

Klepeis VE,Cornell-Bell A,Trinkaus-Randall V

Sat Dec 01 00:00:00 EST 2001

Bioinformatics

Protein Aliases: alpha 8 connexin; cell surface glycoprotein; connexin 50; Connexin-49; Connexin-50; Cx49; Cx50; gap junction alpha 8; Gap junction alpha-8 protein; gap junction membrane channel protein alpha 8; gap junction membrane channel protein alpha-8; gap junction protein alpha 8 50kDa; gap junction protein, alpha 8, 50kDa; Lens fiber protein MP70; lens intrinsic membrane protein MP70; lens opacity 10; MP38; MP64

Gene Aliases: Aey5; CAE; CAE1; Cnx50; CTRCT1; CX50; CZP1; GJA8; Lop10; MP70

UniProt ID: (Sheep) P55917, (Human) P48165, (Mouse) P28236

Entrez Gene ID: (Sheep) 100170231, (Human) 2703, (Rat) 29601, (Mouse) 14616

Molecular Function: gap junction

Function(s)

lens development in camera-type eye transport cell-cell signaling visual perception protein homooligomerization transmembrane transport camera-type eye development cell communication

Process(es)

gap junction channel activity channel activity protein binding

Connexin 50 Monoclonal Antibody (C6)

Product Details

33-4300

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

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