|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rabbit, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of the mouse, rat, Chinese hamster and golden hamster connexin 57 (Cx57) proteins|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
40-5000 was used in immunofluorescence analysis of Connexin57 (Cx57) on sections of adult mouse retina.
This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Connexin 57 is expressed by the axon terminal network of B-type horizontal cells in the rabbit retina.
40-5000 was used in immunohistochemistry to study the expression of connexin 57 in rabbit retina B-type horizontal cells
|Pan F,Keung J,Kim IB,Snuggs MB,Mills SL,O'Brien J,Massey SC||The Journal of comparative neurology (520:2256)||2012|
|Mouse||1:100-1:250||Lack of photoreceptor signaling alters the expression of specific synaptic proteins in the retina.||Kihara AH,Santos TO,Paschon V,Matos RJ,Britto LR||Neuroscience (151:995)||2008|
|Mouse||Not Cited||Connexin-mediated communication controls cell proliferation and is essential in retinal histogenesis.||Kihara AH,Santos TO,Osuna-Melo EJ,Paschon V,Vidal KS,Akamine PS,Castro LM,Resende RR,Hamassaki DE,Britto LR||International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience (28:39)||2010|
|Not Applicable||2 µg/ml||
Spatial relationships of connexin36, connexin57 and zonula occludens-1 in the outer plexiform layer of mouse retina.
40-5000 was used in immunohistochemistry and western blot to investigate connexin57 expression in the mouse retina
|Ciolofan C,Lynn BD,Wellershaus K,Willecke K,Nagy JI||Neuroscience (148:473)||2007|