Western blot analysis of Copine I using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of 3xFlag-human CPNE1-transfected 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Copine I polyclonal antibody (Product # PA5-27412) at a dilution of 1:5000.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 180 of Human Copine I|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27412 targets Copine I in WB applications and shows reactivity with Human and mouse samples.
The PA5-27412 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 180 of Human Copine I.
Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins.
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