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|Tested species reactivity||Virus|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody also reacts with feline infectious peritonitis virus type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissible gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV). Clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcvirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine parvovirus and Canine Parainfluenza virus.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. In Western blot, this antibody detects a band at ~50-56 kDa under reducing conditions, representing the nucleocapsid. Band are present at ~56 and ~46 kDa in reducing gels using a CCV antigen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BCV; bovine coronavirus; canine coronavirus; CCV; coronavirus nucleocapsid; ferret coronavirus; pig coronavirus; swine coronavirus; TGEV; transmissible gastroenteritis virus