|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Tested Species reactivity||Virus|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This antibody also reacts with feline infectious peritonitis virus type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissible gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV). Clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcvirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine parvovirus and Canine Parainfluenza virus.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. In Western blot, this antibody detects a band at ~50-56 kDa under reducing conditions, representing the nucleocapsid. Band are present at ~56 and ~46 kDa in reducing gels using a CCV antigen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BCV; Bovine coronavirus; Canine coronavirus; CCV; Coronavirus nucleocapsid; Feline coronavirus; Ferret coronavirus; Mouse coronavirus; Pig coronavirus; Swine coronavirus; TGEV; Transmissible gastroenteritis virus
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.