This antibody also reacts with feline infectious peritonitis virus type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissible gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV). Clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcvirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine parvovirus and Canine Parainfluenza virus.
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. In Western blot, this antibody detects a band at ~50-56 kDa under reducing conditions, representing the nucleocapsid. Band are present at ~56 and ~46 kDa in reducing gels using a CCV antigen.
Mouse anti Feline Coronavirus antibody, clone FIPV3-70 reacts with feline infectious peritonitis virus (FIPV) type 1 and 2 and is known to be specific for the nucleocapsid.
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Protein Aliases: BCV; Bovine coronavirus; Canine coronavirus; CCV; Feline coronavirus; Ferret coronavirus; Mouse coronavirus; Pig coronavirus; Swine coronavirus; TGEV