Invitrogen

Coronin 1A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (21) Coronin 1A antibodies

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Datasheet

Protocols

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Coronin 1A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Coronin 1A was performed using 70% confluent log phase RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Coronin 1A Polyclonal Antibody (Product # PA1-10022) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Coronin 1A Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), Raji (Lane 2), Ramos (Lane 3), HL-60 (Lane 4), RAW 264.7 (Lane 5), A549 (Lane 6), tissue extracts of Mouse Spleen (Lane 7) and Mouse Lung (Lane 8). The blot was probed with Anti-Coronin 1A Polyclonal Antibody (Product # PA1-10022, 1:4000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 51 kDa band corresponding to Coronin 1A was observed across the cell lines and tissues tested except in A549 which is reported to be negative for Coronin 1A expression.

Coronin 1A Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. A higher level of expression of Coronin 1A was observed in Jurkat, Raji, Ramos, HL-60 and RAW 264.7 when compared A549 using Coronin 1A Polyclonal Antibody (Product # PA1-10022) in Western Blot. {RE}

Product Details

PA1-10022

Applications	Tested Dilution	Publications
Western Blot (WB)	1:4,000-1:10,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	View 1 publication 1 publication

Product Specifications
Species Reactivity	Bovine, Human, Mouse, Pig, Rat
Published species	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	C-terminal peptide of human coronin 1a coupled to KLH View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Purification	purified
Storage buffer	whole serum
Contains	5mM sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2082069

Target Information

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The effect of simvastatin on the proteome of detergent-resistant membrane domains: decreases of specific proteins previously related to cytoskeleton regulation, calcium homeostasis and cell fate.

Proteomics

Ponce J,Brea D,Carrascal M,Guirao V,Degregorio-Rocasolano N,Sobrino T,Castillo J,Dávalos A,Gasull T

PA1-10022 was used in immunocytochemistry to investigate the effect of simvastatin on the proteis in detergent-resistant membrane domains

Sat May 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: actin-binding; Clabp coronin; Clipin-A; coronin 1; coronin, actin binding protein, 1A; coronin-1; Coronin-1A; coronin-like actin binding protein; Coronin-like protein A; Coronin-like protein p57; FLJ41407; MGC117380; TACO; Tryptophan aspartate-containing coat protein

Gene Aliases: CLABP; CLIPINA; CORO1; CORO1A; HCORO1; IMD8; Lmb3; p57; TACO

UniProt ID: (Bovine) Q92176, (Human) P31146, (Mouse) O89053, (Rat) Q91ZN1

Entrez Gene ID: (Bovine) 282196, (Human) 11151, (Pig) 100621483, (Mouse) 12721, (Rat) 155151

Molecular Function: non-motor actin binding protein