Invitrogen

Coronin 1A Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

1 Published Figure

2 References

View all (19) Coronin 1A antibodies

Datasheet

Datasheet

Product Image Gallery

Coronin 1A Antibody — Published figure using Coronin 1A polyclonal antibody (Product # PA1-10022)

Coronin 1A Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of Coronin 1A was performed using 70% confluent log phase RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Coronin 1A Polyclonal Antibody (Product # PA1-10022) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Coronin 1A Antibody in Western Blot (WB) — Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of Jurkat (Lane 1), Raji (Lane 2), Ramos (Lane 3), HL-60 (Lane 4), RAW 264.7 (Lane 5), A549 (Lane 6), tissue extracts of Mouse Spleen (Lane 7) and Mouse Lung (Lane 8). The blot was probed with Anti-Coronin 1A Polyclonal Antibody (Product # PA1-10022, 1:4000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 51 kDa band corresponding to Coronin 1A was observed across the cell lines and tissues tested except in A549 which is reported to be negative for Coronin 1A expression.

Coronin 1A Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. A higher level of expression of Coronin 1A was observed in Jurkat, Raji, Ramos, HL-60 and RAW 264.7 when compared A549 using Coronin 1A Polyclonal Antibody (Product # PA1-10022) in Western Blot. {RE}

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Western Blot (WB)

1:4000-1:10,000

Published Applications

Immunocytochemistry (ICC)

See 1 publication below

Immunofluorescence (IF)

See 1 publication below

Product Specifications

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Published species

Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

C-terminal peptide of human coronin 1a coupled to KLH

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 50% glycerol

Contains

5mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2082069

Target Information

Highly conserved coronin family of proteins/actin regulators from WD-repeat superfamily were originally identified in Dictyostelium, wherein, coronin deletions were observed to affect actin-dependent processes, such as phagocytosis, cell motility, and cytokinesis. Coronin-1A (Coro1A) belongs to Type I coronins, almost exclusively expressed in hematopoietic lineages, and plays a key role during T lymphocyte activation, migration, survival, and calcium signaling. Moreover, Coronin-1A has been documented to be dispensable for actin-dependent functions in macrophages. Coro1a-deficient mice have reduced peripheral T cells due to both an impaired survival of naive cells and migrational defects. Both CD4+ and CD8+ T cell subsets are decreased in the periphery of Coro1a-/- mice while thymic cellularity and subpopulations are similar to those observed in wild-type mice. A nonsense mutation in Coro1a has been shown to protect against systemic lupus in a mouse model. Moreover, it has shown that Coro1a is mutated in a thymic egress-deficient mouse strain, but also in a patient with severe combined immunodeficiency (SCID).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: CLABP; Clipin-A; CLIPINA; coronin 1; coronin, actin binding protein, 1A; coronin-1; Coronin-1A; coronin-like actin binding protein; Coronin-like protein A; Coronin-like protein p57; FLJ41407; HCORO1; MGC117380; p57; TACO; Tryptophan aspartate-containing coat protein

Gene Aliases: BOS_22845; CLABP; CLIPINA; CORO1; CORO1A; HCORO1; IMD8; Lmb3; p57; TACO

UniProt ID: (Bovine) Q92176, (Human) P31146, (Mouse) O89053, (Rat) Q91ZN1

Entrez Gene ID: (Bovine) 282196, (Human) 11151, (Mouse) 12721, (Rat) 155151

Molecular Function: actin family cytoskeletal protein cytoskeletal protein non-motor actin binding protein