Invitrogen

Coronin 1B Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (14) Coronin 1B antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Coronin 1B Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Coronin 1B in human prostate and skeletal muscle tissues using Coronin 1B Polyclonal Antibody (Product # PA5-64443). Corresponding CORO1B RNA-seq data are presented for the same tissues.

Coronin 1B Antibody in Western Blot (WB) — Western blot analysis of Coronin 1B in human cell lines MCF-7 and U-251MG using a Coronin 1B Polyclonal Antibody (Product # PA5-64443). Corresponding CORO1B RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Coronin 1B Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Coronin 1B demonstrates antibody specificity. Immunohistochemical analysis of Coronin 1B using anti-Coronin 1B Polyclonal Antibody (Product # PA5-64443), shows significant staining of Coronin 1B in human prostate and shows minimal or weak staining in human skeletal muscle tissues. The relative expression levels of Coronin 1B within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-64443

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:1,000-1:2,500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human Coronin 1B
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2640078

Product Specific Information

Immunogen sequence: AYVPSKQRDL KISRRNVLSD SRPAMAPGSS HLGAPASTTT AAGATPSGSL ARAGEAGKLE EVMQELRALR ALVKEQGDRI CRLEEQLGRM ENGD

Highest antigen sequence identity to the following orthologs: Mouse - 78%, Rat - 76%.

Target Information

Members of the coronin family, such as CORO1B, are WD repeat-containing actin-binding proteins that regulate cell motility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: coronin, actin binding protein, 1B; Coronin-1B; Coronin-2

Gene Aliases: CORO1B; CORONIN-2

UniProt ID: (Human) Q9BR76

Entrez Gene ID: (Human) 57175

Molecular Function: non-motor actin binding protein

Function(s)

cell migration actin cytoskeleton organization ruffle organization regulation of Arp2/3 complex-mediated actin nucleation negative regulation of Arp2/3 complex-mediated actin nucleation endothelial cell chemotaxis cellular response to platelet-derived growth factor stimulus wound healing actin filament bundle assembly negative regulation of smooth muscle cell chemotaxis actin filament branching cell-cell adhesion protein localization to cell leading edge positive regulation of lamellipodium morphogenesis

Process(es)

protein binding identical protein binding actin filament binding Arp2/3 complex binding cadherin binding involved in cell-cell adhesion

Coronin 1B Polyclonal Antibody

Product Details

PA5-64443

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!