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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 16 and 209 of Human Creatine kinase|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
PA5-27509 targets Creatine kinase (brain) in IF, IHC (P), and WB applications and shows reactivity with Human and mouse samples.
The PA5-27509 immunogen is recombinant fragment corresponding to a region within amino acids 16 and 209 of Human Creatine kinase.
The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Label-free proteomics uncovers energy metabolism and focal adhesion regulations responsive for endometrium receptivity.
PA5-27509 was used in immunohistochemistry - frozen section and western blot to analyze the proteome during the endometrial receptivity process
|Chen Q,Zhang A,Yu F,Gao J,Liu Y,Yu C,Zhou H,Xu C||Journal of proteome research (14:1831)||2015|
CKBB; creatine kinase B chain; creatine kinase B-type; creatine kinase brain; creatine kinase brain-type; creatine kinase, brain; creatine kinase-B; epididymis luminal protein 211; epididymis secretory protein Li 29
B-CK; BCK; Ck-3; Ck3; CKB; CKBB; HEL-211; HEL-S-29