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|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes an epitope located in the C-terminal domain of Csk-binding protein, a 46 kDa ubiquitously expressed transmembrane adaptor protein which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule.
A suggested positive control for Immunoprecipitation is RAJI human burkitt lymphoma cell line. A suggested positive control for immunohistochemistry is appendix (germinal center of lymphatic follicle). For IHC staining, heat-mediated antigen retrieval in citrate buffer, pH 6.1, is recommended.
PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Csk binding protein; Csk-binding protein; phosphoprotein associated with glycosphingolipid microdomains 1; phosphoprotein associated with glycosphingolipid-enriched microdomains 1; phosphoprotein transmembrane adaptor 1; phosphoprotein-associated with GEMs; transmembrane adapter protein PAG; transmembrane adaptor protein PAG; transmembrane phosphoprotein Cbp
CBP; F730007C19Rik; PAG; PAG1