Invitrogen

CtBP2 Recombinant Rabbit Monoclonal Antibody (1R1U6)

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Protocols

Questions & Answers

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CtBP2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of CtBP2 in NIH/3T3 cells using a CtBP2 Recombinant Monoclonal antibody (Product # MA5-42598). Blue: DAPI for nuclear staining.

CtBP2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CtBP2 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42598) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

CtBP2 Antibody in Western Blot (WB) — Western Blot analysis of CtBP2 in various cell lines using a CtBP2 Recombinant Monoclonal antibody (Product # MA5-42598) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.

Product Details

MA5-42598

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500 - 1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-
Immunoprecipitation (IP)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	1R1U6
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 346-445 of human CTBP2 (P56545). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2911739

Product Specific Information

Positive test controls include: HeLa, NIH/3T3, Rat testis. The target is usually found in the following locations: Cell junction, Nucleus, synapse.

Target Information

This gene produces alternative transcripts encoding two distinct proteins. One protein is a transcriptional repressor, while the other isoform is a major component of specialized synapses known as synaptic ribbons. Both proteins contain a NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases. A portion of the 3' untranslated region was used to map this gene to chromosome 21q21. 3; however, it was noted that similar loci elsewhere in the genome are likely. Blast analysis shows that this gene is present on chromosome 10.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C-terminal-binding protein 2; CtBP2; gene trap ROSA b-geo 6; ribeye; RP11-59C5.2

Gene Aliases: AA407280; CTBP2; D7Ertd45e; Gtrgeo6; Ribeye

UniProt ID: (Human) P56545, (Rat) Q9EQH5, (Mouse) P56546

Entrez Gene ID: (Human) 1488, (Rat) 81717, (Mouse) 13017

Molecular Function: transcription cofactor

Function(s)

negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated negative regulation of cell proliferation viral genome replication positive regulation of chromatin binding negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of retinoic acid receptor signaling pathway white fat cell differentiation oxidation-reduction process regulation of transcription, DNA-templated metabolic process cell differentiation

Process(es)

chromatin binding transcription coactivator activity transcription corepressor activity protein binding oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor protein homodimerization activity retinoic acid receptor binding NAD binding oxidoreductase activity

CtBP2 Recombinant Rabbit Monoclonal Antibody (1R1U6)

Product Details

MA5-42598

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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