|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C-D(742) G E K D T Y S Y L A(752) of human Cullin 1.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5-10 µg/ml|
|Immunoprecipitation (IP)||10 µg/mg|
|Western Blot (WB)||5-10 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21787 detects Cullin 1 in human and mouse samples. This antibody does not cross-react with rat Cullin 1.
PA1-21787 has been successfully used in immunohistochemistry (FFPE), immunoprecipitation and Western Blot procedures. No special pretreatment is required for immunostaining of formalin-fixed paraffin-embedded tissue sections.
The PA1-21787 immunogen is a synthetic peptide corresponding to residues C-D(742) G E K D T Y S Y L A(752) of human Cullin 1.
Cullin 1 is a member of the family of human cullin genes (CUL 1, 2, 3, 4a, 4b, and 5) homologous to the S. cerevisiae cdc 53 gene. CUL 1 forms a complex with human p19 skp1 and F box protein p45 skp2, both of which, together with cdc 53 (known as CUL A), as three subunits form a ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The CUL 1/p19 skp1/p45 skp2complex is thought to play a role in the ubiquitination of G1 regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.