This antibody is predicted to react with bovine, canine, chimpanzee, equine, orangutan, primate, Rhesus monkey and Xenopus based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
In yeast, proteolysis of G1 cell cycle regulatory proteins is controlled by an ubiquitin ligase formed by three subunits: Cdc53 (also known as CulA), Skp1 and one of many F-box proteins. A family of human cullin genes homologous to the S. cerevisiae cdc53 gene has been identified (cul1, 2, 3, 4a, 4b, and 5). In humans Cul2 is part of the ECS (Elongin B/C-Cul2/Cul5-SOCS-box protein) complex which contains the Cullin-RBX module. Human Cul2 does not associate with Skp1 and Skp2. Instead it forms a complex with an inactive transcriptional elongation complex, SIII, formed by three subunits: Elongin C, Elongin B and VHL. The Cul2-Rbx1 module specifically interacts with the VHL box. Intriguingly, Elongin C shows homology with Skp1 and Elongin B with ubiquitin. In its active form, SIII does not contain VHL but rather contains an F box protein, Elongin A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CUL-2; Cullin-2; testis secretory sperm-binding protein Li 238E
Gene Aliases: 1300003D18Rik; 4932411N15Rik; AI327301; CUL2; mKIAA4106