Invitrogen

Cyclin A2 Monoclonal Antibody (6B4D11)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

View all (25) Cyclin A2 antibodies

Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Cyclin A2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of CCNA2 was performed using 70% confluent log phase HeLa cells treated with 0.1 µg/mL of nocodazole for 16 h followed by 4h release. The cell were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with a CCNA2 Mouse Monoclonal Antibody (Product # MA1-180) at a dilution of 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with no signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Cyclin A2 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), COLO 205 (Lane 2), NIH3T3 (Lane 3), NIH3T3 treated with 0.1 µg/mL nocadazole for 16 hours (Lane 4) and NIH3T3 treated with 0.1 µg/mL of nocodazole for 16 hours and released for 4 hours. The blots were probed with a CCNA2 Mouse Monoclonal Antibody (Product # MA1-180,) at a dilution of 1-2 µg/mL and detected by chemiluminescence using a Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177) at a dilution of 1:2500. A ~49kDa band corresponding to CCNA2 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with the iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5% Milk in TBST. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Cyclin A2 Antibody — The specificity of anti-Cyclin A2 antibody (Product # MA1-180)) was demonstrated by the western blot detection of decreased expression of Cyclin A2 in NIH3T3 cells upon treatment with Nocodazole compared to untreated cells. {TM}

Product Details

MA1-180

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunocytochemistry (ICC/IF)	1-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	6B4D11
Immunogen	Human CCNA2 expressed in E. Coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 1mg/mL BSA, 30% glycerol
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2633305

Product Specific Information

MA1-180 detects CCNA2 from human and mouse samples.

MA1-180 has been successfully used in western blot and immunofluorescence applications.

Target Information

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.