Western blot analysis was performed on whole cell extracts (30 ug lysate) of K562 (Lane 1), HEL 92.1.7 (Lane 2), HEL 92.1.7 treated with 1uM doxorubicin for 18 hour (Lane 3), HEL 92.1.7 treated with 3uM nocodazole for 24 hours (Lane 4),HeLa (Lane 5), HeLa treated with 1uM doxorubicin for 18 hour (Lane 6).The blots were probed with Anti-Cyclin B1 Mouse Monoclonal Antibody (Product# MA514319, 1-2 ug/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 626520, 1:4000 dilution). A 52 kDa band corresponding to Cyclin B1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Hamster, Human, Mouse|
|Published species reactivity||Bovine, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human cyclin B1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14319 targets Cyclin B1 in FACS, IF, IP, and WB applications and shows reactivity with Hamster, Human, and mouse samples.
The MA5-14319 immunogen is human cyclin B1 protein.
In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mechanism of APC/CCDC20 activation by mitotic phosphorylation.
MA5-14319 was used in western blot to study the mechanism responsible for the activation of mitotic phosphorylation by APC/CCDC20
|Qiao R,Weissmann F,Yamaguchi M,Brown NG,VanderLinden R,Imre R,Jarvis MA,Brunner MR,Davidson IF,Litos G,Haselbach D,Mechtler K,Stark H,Schulman BA,Peters JM||Proceedings of the National Academy of Sciences of the United States of America (113:E2570)||2016|
|Not Applicable||Not Cited||
Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway.
MA5-14319 was used in western blot to determine how the N-end rule pathway degrades separase-cleaved Rec8, a meiotic cohesion subunit
|Liu YJ,Liu C,Chang Z,Wadas B,Brower CS,Song ZH,Xu ZL,Shang YL,Liu WX,Wang LN,Dong W,Varshavsky A,Hu RG,Li W||The Journal of biological chemistry (291:7426)||2016|
Cyclin A degradation by primate cytomegalovirus protein pUL21a counters its innate restriction of virus replication.
MA5-14319 was used in western blot to study the ability of primate CMV pUL21a to degrade cyclin A and the implications for virus replication
|Caffarelli N,Fehr AR,Yu D||PLoS pathogens (9:null)||2014|
Prenylated Flavonoids from Morus alba L. Cause Inhibition of G1/S Transition in THP-1 Human Leukemia Cells and Prevent the Lipopolysaccharide-Induced Inflammatory Response.
MA5-14319 was used in western blot to study the anti-proliferative and anti-inflammatory effects of three Morus alba L. flavonoids on human THP-1 leukemia cells
|Kollar P,Bárta T,Ho¿ek J,Sou¿ek K,Závalová VM,Artinian S,Talhouk R,Smejkal K,Suchý P,Hampl A||Evidence-based complementary and alternative medicine : eCAM (2013:null)||2013|
Proteasome-dependent disruption of the E3 ubiquitin ligase anaphase-promoting complex by HCMV protein pUL21a.
MA5-14319 was used in western blot to study the role of the proteasome in the disruption of the anaphase-promoting complex by the pUL21a protein of human CMV
|Fehr AR,Gualberto NC,Savaryn JP,Terhune SS,Yu D||PLoS pathogens (8:null)||2012|
Chemical-genetic analysis of cyclin dependent kinase 2 function reveals an important role in cellular transformation by multiple oncogenic pathways.
MA5-14319 was used in western blot to study the role of CDK2 in multiple oncogenic pathways as revealed by a combined siRNA and small molecule inhibitor approach
|Horiuchi D,Huskey NE,Kusdra L,Wohlbold L,Merrick KA,Zhang C,Creasman KJ,Shokat KM,Fisher RP,Goga A||Proceedings of the National Academy of Sciences of the United States of America (109:E1019)||2012|
|Not Applicable||Not Cited||
Synergistic Effect of Hyperglycemia and p27(kip1) Suppression on Adult Mouse Islet Beta Cell Replication.
MA5-14319 was used in western blot to explore the contributions of hyperglycemia and p27(kip1) suppression on islet beta cell regeneration
|Chen ST,Fu SH,Hsu S,Huang YY,Hsu BR||International journal of endocrinology (2012:null)||2012|
CDK1, not ERK1/2 or ERK5, is required for mitotic phosphorylation of BIMEL.
MA5-14319 was used in western blot to study the mitotic phosphorylation of BIMEL and the essential role of CDK1
|Gilley R,Lochhead PA,Balmanno K,Oxley D,Clark J,Cook SJ||Cellular signalling (24:170)||2012|
Possible role of 5'AMP-activated protein kinase in the metformin-mediated arrest of bovine oocytes at the germinal vesicle stage during in vitro maturation.
MA5-14319 was used in western blot to study the potential role of 5'AMP-activated protein kinase in the metformin-mediated arrest of bovine oocyte maturation
|Tosca L,Uzbekova S,Chabrolle C,Dupont J||Biology of reproduction (77:452)||2007|
Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells.
MA5-14319 was used in western blot to study the role of p21(Cip1) and p27(Kip1) in the induction of cell cycle arrest in human panceatic adenocarcinoma cells by isoprenoids
|Wiseman DA,Werner SR,Crowell PL||The Journal of pharmacology and experimental therapeutics (320:1163)||2007|
Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes.
MA5-14319 was used in western blot to study the role of cytoplasmically retained active Cdk1/cyclin B1 complex in G2 cell cycle arrest induced by HPV
|Davy CE,Jackson DJ,Raj K,Peh WL,Southern SA,Das P,Sorathia R,Laskey P,Middleton K,Nakahara T,Wang Q,Masterson PJ,Lambert PF,Cuthill S,Millar JB,Doorbar J||Journal of virology (79:3998)||2005|
HYAL1 hyaluronidase: a molecular determinant of bladder tumor growth and invasion.
MA5-14319 was used in western blot to investigate the role of HYAL1 hyaluronidase in bladder tumor growth and metastases
|Lokeshwar VB,Cerwinka WH,Lokeshwar BL||Cancer research (65:2243)||2005|
c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells.
MA5-14319 was used in western blot to study the effect of c-Myc on DNA damage-induced G2/M arrest.
|Sheen JH,Woo JK,Dickson RB||British journal of cancer (89:1479)||2003|
NB1011 induces Ser15 phosphorylation of p53 and activates the G2/M checkpoint.
MA5-14319 was used in western blot to study the ability of a novel anti-cancer molecule to induce phosphorylation of p53 and inhibit growth of a breast carcinoma cell line by G2/M checkpoint activation
|Dellinger RW,Karjian PL,Neuteboom ST||Anti-cancer drugs (14:449)||2003|
Repression of cyclin D1 expression is necessary for the maintenance of cell cycle exit in adult mammalian cardiomyocytes.
MA5-14319 was used in immunoprecipitation to study the role of cyclin D1 in adult mammalian cardiomyocyte cell cycle exit
|Tane S,Kubota M,Okayama H,Ikenishi A,Yoshitome S,Iwamoto N,Satoh Y,Kusakabe A,Ogawa S,Kanai A,Molkentin JD,Nakamura K,Ohbayashi T,Takeuchi T||The Journal of biological chemistry (289:18033)||2014|
Cell cycle regulation in mouse heart during embryonic and postnatal stages.
MA5-14319 was used in immunoprecipitation to study the molecular biology of cell cycle regulation in embryonic and post-natal murine heart
|Ikenishi A,Okayama H,Iwamoto N,Yoshitome S,Tane S,Nakamura K,Obayashi T,Hayashi T,Takeuchi T||Development, growth and differentiation (54:731)||2012|
Mechanisms governing maintenance of Cdk1/cyclin B1 kinase activity in cells infected with human cytomegalovirus.
MA5-14319 was used in immunoprecipitation to investigate Cdk1/cyclin B1 activity in HCMV-infected cells
|Sanchez V,McElroy AK,Spector DH||Journal of virology (77:13214)||2003|
A cyclin-binding motif in human papillomavirus type 18 (HPV18) E1^E4 is necessary for association with CDK-cyclin complexes and G2/M cell cycle arrest of keratinocytes, but is not required for differentiation-dependent viral genome amplification or L1 capsid protein expression.
MA5-14319 was used in immunocytochemistry to study the role of a HPV18 cyclin-binding motif in CDK-cyclin complex association and G2/M cell cycle arrest in keratinocytes
|Knight GL,Pugh AG,Yates E,Bell I,Wilson R,Moody CA,Laimins LA,Roberts S||Virology (412:196)||2011|
Mitotic checkpoint slippage in humans occurs via cyclin B destruction in the presence of an active checkpoint.
MA5-14319 was used in immunocytochemistry to study the role of cyclin B destruction in mitotic checkpoint slippage in humans
|Brito DA,Rieder CL||Current biology : CB (16:1194)||2006|