Western blot analysis of Cyclin B1 was performed by loading 25 ug of HT29 (Lane 1) and Jurkat (Lane 2)cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Cyclin B1 monoclonal antibody (Product # MA5-14327) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 58 kDa.
|Tested species reactivity||Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human cyclin B1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14324 targets Cyclin B1 in ICC/IF, IHC (P), WB and IP applications and shows reactivity with Hamster, Human, mouse, and Rat samples.
The MA5-14324 immunogen is human cyclin B1 protein.
In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MYC expression and distribution in normal mature lymphoid cells.
MA5-14324 was used in western blot to study which normal mature lymphoid cells express myc
|Cattoretti G||The Journal of pathology (229:430)||2013|
Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post-mitotic newborn neurons.
MA5-14324 was used in immunohistochemistry to develop a high resolution map of the kinetics of differentiation of murine retinal newborn neurons
|Pacal M,Bremner R||Developmental dynamics : an official publication of the American Association of Anatomists (241:1525)||2012|
Cyclin A and cyclin B1 overexpression in differentiated thyroid carcinoma.
MA5-14324 was used in immunohistochemistry to study the diagnostic value of cyclin A and cyclin D1 expression in differentiated thyroid carcinoma
|Nar A,Ozen O,Tutuncu NB,Demirhan B||Medical oncology (Northwood, London, England) (29:294)||2012|
Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis.
MA5-14324 was used in immunohistochemistry to study the immunohistochemistry of non-small cell lung cancer
|Yoo J,Jung JH,Lee MA,Seo KJ,Shim BY,Kim SH,Cho DG,Ahn MI,Kim CH,Cho KD,Kang SJ,Kim HK||Journal of Korean medical science (22:318)||2007|
Mitotic checkpoint slippage in humans occurs via cyclin B destruction in the presence of an active checkpoint.
MA5-14324 was used in immunocytochemistry to study the role of cyclin B destruction in mitotic checkpoint slippage in humans
|Brito DA,Rieder CL||Current biology : CB (16:1194)||2006|
Mitotic cell cycle proteins increase in podocytes despite lack of proliferation.
MA5-14324 was used in immunocytochemistry to study the increase in mitotic cell cycle protein in podocyes in the absence of increased proliferation
|Petermann AT,Pippin J,Hiromura K,Monkawa T,Durvasula R,Couser WG,Kopp J,Shankland SJ||Kidney international (63:113)||2003|
Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes.
MA5-14324 was used in immunoprecipitation to study the role of cytoplasmically retained active Cdk1/cyclin B1 complex in G2 cell cycle arrest induced by HPV
|Davy CE,Jackson DJ,Raj K,Peh WL,Southern SA,Das P,Sorathia R,Laskey P,Middleton K,Nakahara T,Wang Q,Masterson PJ,Lambert PF,Cuthill S,Millar JB,Doorbar J||Journal of virology (79:3998)||2005|