|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:100-1:1000|
|Immunocytochemistry (ICC)||See 2 publications below|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human cyclin B1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-14327 targets Cyclin B1 in WB, ICC/IF, IHC (P), and IP applications and shows reactivity with Hamster, Human, mouse, and Rat samples. This antibody is not recommended for NIH-3T3 cells in Western blot applications.
The MA5-14327 immunogen is human cyclin B1 protein.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cyclin B1; cyclin B1, related sequence 1; cyclin B1, related sequence 13; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1; OTTHUMP00000223023
Gene Aliases: Ccn-2; CCNB; CCNB1; Ccnb1-rs1; Ccnb1-rs13; Cycb; Cycb-4; Cycb-5; CycB1; Cycb1-rs1; I79_013589
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