Immunofluorescent analysis of Cyclin B1 (green) in HeLa cells either left untreated (left panel) or treated with 50nM Nocodazole (right panel) for 16 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Cyclin B1 monoclonal antibody (Product # MA5-14327) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan or ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Hamster, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human cyclin B1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14327 targets Cyclin B1 in WB, ICC/IF, IHC (P), and IP applications and shows reactivity with Hamster, Human, mouse, and Rat samples. This antibody is not recommended for NIH-3T3 cells in Western blot applications.
The MA5-14327 immunogen is human cyclin B1 protein.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
In mammals, cyclin B associates with inactive p34cdc2. Cyclin B-p34cdc2 plays a critical role in G2 to M transition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
The Interaction between Cyclin B1 and Cytomegalovirus Protein Kinase pUL97 is Determined by an Active Kinase Domain.
MA5-14327 was used in immunocytochemistry and western blot to study the interaction between cytomegalovirus protein kinase pUL97 and Cyclin B1 determined by an active kinase domain
|Steingruber M,Socher E,Hutterer C,Webel R,Bergbrede T,Lenac T,Sticht H,Marschall M||Viruses (7:4582)||2015|
Mitotic cell cycle proteins increase in podocytes despite lack of proliferation.
MA5-14327 was used in immunocytochemistry to study the increase in mitotic cell cycle protein in podocyes in the absence of increased proliferation
|Petermann AT,Pippin J,Hiromura K,Monkawa T,Durvasula R,Couser WG,Kopp J,Shankland SJ||Kidney international (63:113)||2003|
MYC expression and distribution in normal mature lymphoid cells.
MA5-14327 was used in western blot to study which normal mature lymphoid cells express myc
|Cattoretti G||The Journal of pathology (229:430)||2013|
Mapping differentiation kinetics in the mouse retina reveals an extensive period of cell cycle protein expression in post-mitotic newborn neurons.
MA5-14327 was used in immunohistochemistry to develop a high resolution map of the kinetics of differentiation of murine retinal newborn neurons
|Pacal M,Bremner R||Developmental dynamics : an official publication of the American Association of Anatomists (241:1525)||2012|
Cyclin A and cyclin B1 overexpression in differentiated thyroid carcinoma.
MA5-14327 was used in immunohistochemistry to study the diagnostic value of cyclin A and cyclin D1 expression in differentiated thyroid carcinoma
|Nar A,Ozen O,Tutuncu NB,Demirhan B||Medical oncology (Northwood, London, England) (29:294)||2012|
Immunohistochemical analysis of non-small cell lung cancer: correlation with clinical parameters and prognosis.
MA5-14327 was used in immunohistochemistry to study the immunohistochemistry of non-small cell lung cancer
|Yoo J,Jung JH,Lee MA,Seo KJ,Shim BY,Kim SH,Cho DG,Ahn MI,Kim CH,Cho KD,Kang SJ,Kim HK||Journal of Korean medical science (22:318)||2007|
Human papillomavirus type 16 E1 E4-induced G2 arrest is associated with cytoplasmic retention of active Cdk1/cyclin B1 complexes.
MA5-14327 was used in immunoprecipitation to study the role of cytoplasmically retained active Cdk1/cyclin B1 complex in G2 cell cycle arrest induced by HPV
|Davy CE,Jackson DJ,Raj K,Peh WL,Southern SA,Das P,Sorathia R,Laskey P,Middleton K,Nakahara T,Wang Q,Masterson PJ,Lambert PF,Cuthill S,Millar JB,Doorbar J||Journal of virology (79:3998)||2005|