Immunofluorescent analysis of Cyclin B2 (green) in asynchronous HeLa cells showing cytoplasmic and nuclear localization. Images shown are without (left panel) or with (right panel) nuclear Hoechst staining. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Cyclin B2 monoclonal antibody (Product # MA1-156) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502). Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249) for 30 minutes. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Xenopus laevis cyclin B2|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-156 detects Cyclin B2 in mammalian samples. MA1-156 has been successfully used in Immunofluorescence, Immunoprecipitation, IHC (P) and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-156 show the accumulation of a prominent band at ~51 kDa in camptothecin and hydroxyurea treated cells. MA1-156 also detects additional unknown band at ~80 kDa. In Immunofluorescence applications, MA1-156 shows cyclin B2 staining consistent with the Golgi region, whereas MA1-155 shows cyclin B1 co-localization with microtubules.
MA1-156 reacts with Cyclin B2 from Xenopus laevis and mammalian sources. In Western blot applications, X29.2 also cross reacts with Cyclin B1.
Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). The protein encoded by the CCNB2 gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Cyclin B2 steadily accumulates during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B2 is required for cell cycle progression, as destruction resistant mutants cause mitotic arrest. Cyclins B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.