Bioss

Cyclin B2 Monoclonal Antibody (2F4)

Product Image Gallery

Cyclin B2 Antibody in Western Blot (WB) — Lane 1: Hela; Lane 2: K562 lysates probed with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:1000 overnight at 4°C. Followed by a conjugated secondary antibody.

Cyclin B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Paraformaldehyde-fixed, paraffin embedded mouse testis section; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal serum) at 37°C for 20min; Antibody incubation with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at 1:50 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Cyclin B2 Antibody in Immunofluorescence (IF) — PC12 cells were stained with Cyclin B2 (2F4) Monoclonal Antibody (bsm-52045R) at [1:200] incubated overnight at 4C, followed by secondary antibody incubation, DAPI staining of the nuclei and detection.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Immunofluorescence (IF)

1:200

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

2F4

Immunogen

Recombinant human Cyclin B2 protein, around N-terminal 150aa.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS with 1% BSA, 40% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Target Information

Cyclin B2 is a member of the cyclin family, specifically the B-type cyclins. The B-type cyclins, B1 and B2, associate with p34cdc2 and are essential components of the cell cycle regulatory machinery. B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region. Cyclin B2 also binds to transforming growth factor beta RII and thus cyclin B2/cdc2 may play a key role in transforming growth factor beta-mediated cell cycle control.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.