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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant full-length human cyclin D1 protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunohistochemistry (IHC)||2-10 ug/ml|
|Immunoprecipitation (IP)||5 ug|
|Western Blot (WB)||0.3-1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Cyclin D1 (Bcl-1 oncogene product), is a member of the Cyclin family that help control transition between phases of the cell cycle by acting as regulatory subunits of the Cdks. The D-type Cyclins are composed of three subtypes, Cyclin D1, D2 and D3. Cyclin D1 is essential for control of the cell cycle at the G1/S transitions and interacts with Cdk4 or Cdk6 protein kinases. On the recent study, Cyclin D1 regulates cellular metabolism, fat cell differentiation and cellular migration. Also Cyclin D1 involves in development and cancer.
IP-MS enrichment of CCND1 (LFQ intensity): CCND1 was enriched 5-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and CCND1 antibody (Part No. 33-3500). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||Cell cycle regulation of human endometrial stromal cells during decidualization.||Logan PC,Steiner M,Ponnampalam AP,Mitchell MD||Reproductive sciences (Thousand Oaks, Calif.) (19:883)||2012|
Regulation of cranial morphogenesis and cell fate at the neural crest-mesoderm boundary by engrailed 1.
33-3500 was used in immunohistochemistry - paraffin section to determine the mesenchymal precursors that generate the coronal suture.
|Deckelbaum RA,Holmes G,Zhao Z,Tong C,Basilico C,Loomis CA||Development (Cambridge, England) (139:1346)||2012|
|Human||Not Cited||Protein expression profiling identifies subclasses of breast cancer and predicts prognosis.||Jacquemier J,Ginestier C,Rougemont J,Bardou VJ,Charafe-Jauffret E,Geneix J,Adélaïde J,Koki A,Houvenaeghel G,Hassoun J,Maraninchi D,Viens P,Birnbaum D,Bertucci F||Cancer research (65:767)||2005|
B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1 oncogene; BCL1; CCND1; CYL-1; G1/S-specific cyclin D1; G1/S-specific cyclin-D1; PRAD1; PRAD1 oncogene
AI327039; bcl-1; BCL1; CCND1; cD1; CycD1; Cyl-1; D11S287E; PRAD1; U21B31