|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full length fusion protein (human)|
|Storage buffer||whole serum|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-31443 detects Cyclin D1 from rat, mouse, human samples.
PA1-31443 has been successfully used in Western blot and ELISA applications.
The PA1-31443 immunogen is Full length fusion protein (human).
During each cell cycle cyclins undergo periodic accumulation an destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks. Early in the G1 phase of cell cycle cyclin D1 induction is followed by cyclin E induction. The sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition to S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.