|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:25|
|Immunohistochemistry (IHC)||See 151 publications below|
|Western Blot (WB)||See 20 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 12 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from C-terminus of human cyclin D1.|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma or mantle cell lymphoma.
Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; BCL1; D11S287E; G1/S-specific cyclin-D1; PRAD1; PRAD1 oncogene; U21B31
Gene Aliases: BCL1; CCND1; D11S287E; PRAD1; U21B31
UniProt ID: (Human) P24385
Entrez Gene ID: (Human) 595
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