|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human recombinant full length cyclin D1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Functional Assay (FN)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12707 targets Cyclin D1/Bcl-1 in FN applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-12707 immunogen is human recombinant full length cyclin D1 protein.
Cyclin D1or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL)
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point.
MA5-12707 was used in immunoprecipitation to study the roles CDK7 and p21 in regulating the T172 phosphorylation of CDK4 at the restriction point
|Bisteau X,Paternot S,Colleoni B,Ecker K,Coulonval K,De Groote P,Declercq W,Hengst L,Roger PP||PLoS genetics (9:null)||2013|
Cyclic AMP inhibits the proliferation of thyroid carcinoma cell lines through regulation of CDK4 phosphorylation.
MA5-12707 was used in immunoprecipitation to study the role of CDK4 phosphorylation in the mechanism by which cAMP inhibits thyroid carcinoma cell proliferation
|Rocha AS,Paternot S,Coulonval K,Dumont JE,Soares P,Roger PP||Molecular biology of the cell (19:4814)||2008|
Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.
MA5-12707 was used in immunoprecipitation to study the mechanism by which the nuclear receptor family member SHP suppresses hepatic carcinogenesis
|Zhang Y,Xu P,Park K,Choi Y,Moore DD,Wang L||Hepatology (Baltimore, Md.) (48:289)||2008|
p21Cip1 and p27Kip1 induce distinct cell cycle effects and differentiation programs in myeloid leukemia cells.
MA5-12707 was used in immunoprecipitation to study the role of p21Cip1 and p27Kip1 in the cell cycle and differentiation programs of myeloid leukemia cells
|Muñoz-Alonso MJ,Acosta JC,Richard C,Delgado MD,Sedivy J,León J||The Journal of biological chemistry (280:18120)||2005|
Cyclin C/cdk3 promotes Rb-dependent G0 exit.
MA5-12707 was used in immunoprecipitation to study the role of cyclin C/cdk3 in promoting Rb-dependent G0 exit
|Ren S,Rollins BJ||Cell (117:239)||2004|
Differential effects of p21(WAF1/CIP1) deficiency on MMTV-ras and MMTV-myc mammary tumor properties.
MA5-12707 was used in immunoprecipitation to study the role of the CDK inhibitor p21(WAF1/CIP1) in tumor suppression in MMTV-ras and MMTV-myc transgenic mice
|Bearss DJ,Lee RJ,Troyer DA,Pestell RG,Windle JJ||Cancer research (62:2077)||2002|
Metabolic stabilization of p27 in senescent fibroblasts correlates with reduced expression of the F-box protein Skp2.
MA5-12707 was used in immunoprecipitation to study the correlation of p27(KIP1) accumulation in senescent fibroblasts with reduced levels of the F-box protein Skp2
|Wagner M,Hampel B,Hütter E,Pfister G,Krek W,Zwerschke W,Jansen-Dürr P||Experimental gerontology (37:41)||2001|
Reconstitution of cyclin D1-associated kinase activity drives terminally differentiated cells into the cell cycle.
MA5-12707 was used in immunoprecipitation to study the ability of restoring cyclin D1-associated kinase activity to drive terminally differentiated myotubes to enter the cell cycle
|Latella L,Sacco A,Pajalunga D,Tiainen M,Macera D,D'Angelo M,Felici A,Sacchi A,Crescenzi M||Molecular and cellular biology (21:5631)||2001|
Loss of p27Kip1 from cyclin E/cyclin-dependent kinase (CDK) 2 but not from cyclin D1/CDK4 complexes in cells transformed by polyamine biosynthetic enzymes.
MA5-12707 was used in immunoprecipitation to study the role of p27Kip1 downregulation in tumorigenic transformation induced by polyamine biosynthetic enzyme overexpression
|Ravanko K,Järvinen K,Paasinen-Sohns A,Hölttä E||Cancer research (60:5244)||2000|
Inhibition of basal and mitogen-stimulated pancreatic cancer cell growth by cyclin D1 antisense is associated with loss of tumorigenicity and potentiation of cytotoxicity to cisplatinum.
MA5-12707 was used in immunoprecipitation to study the effect of cyclin D1 knockdown on the tumorigenicity and cisplantin-sensitivity of PANC-1 human pancreatic cancer cells
|Kornmann M,Arber N,Korc M||The Journal of clinical investigation (101:344)||1998|
Angiotensin II activation of cyclin D1-dependent kinase activity.
MA5-12707 was used in immunoprecipitation to investigate the effect of angiotensin II on cyclin D1 expression and cyclin D1-dependent kinase activity in H295R cells
|Watanabe G,Lee RJ,Albanese C,Rainey WE,Batlle D,Pestell RG||The Journal of biological chemistry (271:22570)||1996|
Aurora B kinase phosphorylates and instigates degradation of p53.
MA5-12707 was used in western blot to study the degradation of p53 and the accelerating role of phosphorylation by Aurora B kinase
|Gully CP,Velazquez-Torres G,Shin JH,Fuentes-Mattei E,Wang E,Carlock C,Chen J,Rothenberg D,Adams HP,Choi HH,Guma S,Phan L,Chou PC,Su CH,Zhang F,Chen JS,Yang TY,Yeung SC,Lee MH||Proceedings of the National Academy of Sciences of the United States of America (109:E1513)||2012|
Molecular characterization of spontaneous mesenchymal stem cell transformation.
MA5-12707 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation
|Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A||PloS one (3:null)||2008|
Cyclin D1 antagonizes BRCA1 repression of estrogen receptor alpha activity.
MA5-12707 was used in western blot to study the opposing effect of cyclin D1 and estrogen receptor on BRCA1 repression
|Wang C,Fan S,Li Z,Fu M,Rao M,Ma Y,Lisanti MP,Albanese C,Katzenellenbogen BS,Kushner PJ,Weber B,Rosen EM,Pestell RG||Cancer research (65:6557)||2005|
Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts.
MA5-12707 was used in western blot to study senescence-related changes in respiration and oxidative phosphorylation in primary human fibroblasts
|Hutter E,Renner K,Pfister G,Stöckl P,Jansen-Dürr P,Gnaiger E||The Biochemical journal (380:919)||2004|
Progesterone inhibits estrogen-induced cyclin D1 and cdk4 nuclear translocation, cyclin E- and cyclin A-cdk2 kinase activation, and cell proliferation in uterine epithelial cells in mice.
MA5-12707 was used in immunohistochemistry to study the molecular mechanisms underlying the effects of female sex hormones on the proliferation of murine uterine epithelial cells
|Tong W,Pollard JW||Molecular and cellular biology (19:2251)||1999|