Immunohistochemistry analysis of Cyclin D1 / Bcl-1 showing staining in the nucleus and weak cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclin D1 / Bcl-1 Rabbit Polyclonal Antibody (PA516232) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Fish, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the C-terminus of human cyclin D1|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||5-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16232 targets Cyclin D1/Bcl-1 in IF, IP, and WB applications and shows reactivity with mouse, Rat, and Human samples.
The PA5-16232 immunogen is a synthetic peptide derived from the C-terminus of human cyclin D1.
Cyclin D1 (also known as PRAD-1 or bcl-1) is one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. About 50-70% of mantle cell lymphomas (MCL) contain a t (11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1.
IP-MS enrichment of CCND1 (LFQ intensity): CCND1 was enriched 31-fold from HCT116 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and CCND1 antibody (Part No. PA5-16232). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cyclin D1 and C/EBPβ LAP1 operate in a common pathway to promote mammary epithelial cell differentiation.
PA5-16232 was used in ChIP assay and western blot to investigate the relationship between cyclin D1 and the transcription factor C/EBPbeta in mammary epithelial cell differentiation
|Liu Q,Boudot A,Ni J,Hennessey T,Beauparlant SL,Rajabi HN,Zahnow C,Ewen ME||Molecular and cellular biology (34:3168)||2014|
Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase.
PA5-16232 was used in western blot to elucidate how EglN2 regulates FOXO3a degradation.
|Zheng X,Zhai B,Koivunen P,Shin SJ,Lu G,Liu J,Geisen C,Chakraborty AA,Moslehi JJ,Smalley DM,Wei X,Chen X,Chen Z,Beres JM,Zhang J,Tsao JL,Brenner MC,Zhang Y,Fan C,DePinho RA,Paik J,Gygi SP,Kaelin WG,Zhang Q||Genes and development (28:1429)||2014|
p27 is regulated independently of Skp2 in the absence of Cdk2.
PA5-16232 was used in western blot to use knockout mice to study the Skp2-independent modulation of p27 in Cdk2 ablated mice
|Kotoshiba S,Gopinathan L,Pfeiffenberger E,Rahim A,Vardy LA,Nakayama K,Nakayama KI,Kaldis P||Biochimica et biophysica acta (1843:436)||2014|
A melanocyte lineage program confers resistance to MAP kinase pathway inhibition.
PA5-16232 was used in western blot to study MAPK pathway inhibition resistance in malignant melanoma and the role of melanocyte lineage dysregulation
|Johannessen CM,Johnson LA,Piccioni F,Townes A,Frederick DT,Donahue MK,Narayan R,Flaherty KT,Wargo JA,Root DE,Garraway LA||Nature (504:138)||2013|
Cyclin D1 induction of Dicer governs microRNA processing and expression in breast cancer.
PA5-16232 was used in western blot to study miRNA expression and processing in breast cancer and the regulatory role of cyclin D1 induction of Dicer expression
|Yu Z,Wang L,Wang C,Ju X,Wang M,Chen K,Loro E,Li Z,Zhang Y,Wu K,Casimiro MC,Gormley M,Ertel A,Fortina P,Chen Y,Tozeren A,Liu Z,Pestell RG||Nature communications (4:null)||2013|
The oxysterol receptors LXRα and LXRβ suppress proliferation in the colon.
PA5-16232 was used in western blot to study the role of oxysterol receptors LXRalpha and LXRbeta in suppressing proliferation in the colon
|Vedin LL,Gustafsson JÅ,Steffensen KR||Molecular carcinogenesis (52:835)||2013|
Nuclear factor of activated T cells c1 mediates p21-activated kinase 1 activation in the modulation of chemokine-induced human aortic smooth muscle cell F-actin stress fiber formation, migration, and proliferation and injury-induced vascular wall remodeling.
PA5-16232 was used in western blot to study the roles of NFATc1 and Pak1 in chemokine-induced aortic smooth muscle proliferation and vascular remodeling following injury
|Kundumani-Sridharan V,Singh NK,Kumar S,Gadepalli R,Rao GN||The Journal of biological chemistry (288:22150)||2013|
Combined Stimulation with the Tumor Necrosis Factor α and the Epidermal Growth Factor Promotes the Proliferation of Hepatocytes in Rat Liver Cultured Slices.
PA5-16232 was used in western blot to study the proliferation of liver slice hepatocytes in response to combined stimulation with TNF-alpha and EGF
|Finot F,Masson R,Desmots F,Ribault C,Bichet N,Vericat JA,Lafouge P,Guguen-Guillouzo C,Loyer P||International journal of hepatology (2012:null)||2012|
Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling.
PA5-16232 was used in western blot to study the role of protein kinase N1 in NFADc1-dependent cyclin D1/CDK6 expression and activity and the significance for remodeling of inner bood vessel walls
|Singh NK,Kundumani-Sridharan V,Kumar S,Verma SK,Kotla S,Mukai H,Heckle MR,Rao GN||The Journal of biological chemistry (287:36291)||2012|
Cell cycle arrest associated with anoxia-induced quiescence, anoxic preconditioning, and embryonic diapause in embryos of the annual killifish Austrofundulus limnaeus.
PA5-16232 was used in western blot to study the role of cell cycle arrest in Austrofundulus limnaeus embryonic diapause
|Meller CL,Meller R,Simon RP,Culpepper KM,Podrabsky JE||Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology (182:909)||2012|
Modification of the DNA damage response by therapeutic CDK4/6 inhibition.
PA5-16232 was used in western blot to study the effects of therapeutic CDK4/6 inhibition on the DNA damage response and the clinical implications
|Dean JL,McClendon AK,Knudsen ES||The Journal of biological chemistry (287:29075)||2012|
CDK4/6 inhibition antagonizes the cytotoxic response to anthracycline therapy.
PA5-16232 was used in western blot to study the protective effect of pharmacological inhibition of CDK4/6 against antracycline cytotoxicity in triple-negative breast cancer
|McClendon AK,Dean JL,Rivadeneira DB,Yu JE,Reed CA,Gao E,Farber JL,Force T,Koch WJ,Knudsen ES||Cell cycle (Georgetown, Tex.) (11:2747)||2012|
MUC1-C oncoprotein induces TCF7L2 transcription factor activation and promotes cyclin D1 expression in human breast cancer cells.
PA5-16232 was used in western blot to study the role of TCF7L2 activation in the mechanism by which oncogenic MUC1-C promotes human breast cancer cell cyclin D1 expression
|Rajabi H,Ahmad R,Jin C,Kosugi M,Alam M,Joshi MD,Kufe D||The Journal of biological chemistry (287:10703)||2012|
Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells.
PA5-16232 was used in western blot to study the role of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway in the transformation of breast tumor cells
|Sarkar TR,Sharan S,Wang J,Pawar SA,Cantwell CA,Johnson PF,Morrison DK,Wang JM,Sterneck E||Molecular and cellular biology (32:320)||2012|
Low-oxygen culture conditions extend the multipotent properties of human retinal progenitor cells.
PA5-16232 was used in immunohistochemistry to study the extension of human retinal progenitor cell multipotency and self-renewal by culture under conditions of low oxygen
|Baranov PY,Tucker BA,Young MJ||Tissue engineering. Part A (20:1465)||2014|
The widely used Wnt1-Cre transgene causes developmental phenotypes by ectopic activation of Wnt signaling.
PA5-16232 was used in immunohistochemistry to evaluate the Wnt1-Cre transgenic mouse line as a model for the study of midbrain development
|Lewis AE,Vasudevan HN,O'Neill AK,Soriano P,Bush JO||Developmental biology (379:229)||2013|
Apoptosis signal-regulating kinase-1 inhibitor as a potent therapeutic drug for the treatment of gastric cancer.
PA5-16232 was used in immunohistochemistry to study the therapeutic potential of ASK1 inhibition in gastric cancer
|Hayakawa Y,Hirata Y,Sakitani K,Nakagawa H,Nakata W,Kinoshita H,Takahashi R,Takeda K,Ichijo H,Maeda S,Koike K||Cancer science (103:2181)||2012|
Cyclin D1 inactivation extends proliferation and alters histogenesis in the postnatal mouse retina.
PA5-16232 was used in immunohistochemistry to study post-natal murine retinal histogenesis and the role played by cyclin D1
|Das G,Clark AM,Levine EM||Developmental dynamics : an official publication of the American Association of Anatomists (241:941)||2012|
A microRNA-21 surge facilitates rapid cyclin D1 translation and cell cycle progression in mouse liver regeneration.
PA5-16232 was used in immunohistochemistry to study the role of enhanced cyclin D1 translation in the mechanism by which miRNA-21 promotes liver regeneration
|Ng R,Song G,Roll GR,Frandsen NM,Willenbring H||The Journal of clinical investigation (122:1097)||2012|
Novel interactions between NFATc1 (Nuclear Factor of Activated T cells c1) and STAT-3 (Signal Transducer and Activator of Transcription-3) mediate G protein-coupled receptor agonist, thrombin-induced biphasic expression of cyclin D1, with first phase influencing cell migration and second phase directing cell proliferation.
PA5-16232 was used in immunocytochemistry to study the role of NFATc1 and STAT3 in mediating the expression of cyclin D1
|Kundumani-Sridharan V,Van Quyen D,Subramani J,Singh NK,Chin YE,Rao GN||The Journal of biological chemistry (287:22463)||2012|