Immunohistochemistry analysis of Cyclin D1 / Bcl-1 showing staining in the nucleus and weak cytoplasm of paraffin-embedded mouse liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclin D1 / Bcl-1 Rabbit Polyclonal Antibody (PA516777) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from C-terminus of human cyclin D1|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16777 targets Cyclin D1/Bcl-1 in IHC (P), IP, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The PA5-16777 immunogen is a synthetic peptide from C-terminus of human cyclin D1.
Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Aberrant Activation of the RANK Signaling Receptor Induces Murine Salivary Gland Tumors.
PA5-16777 was used in western blot to study the function of RANKL/RANK signaling pathway during the salivary gland tumorigenicity
|Szwarc MM,Kommagani R,Jacob AP,Dougall WC,Ittmann MM,Lydon JP||PloS one (10:null)||2015|
Chronic Deletion and Acute Knockdown of Parkin Have Differential Responses to Acetaminophen-induced Mitophagy and Liver Injury in Mice.
PA5-16777 was used in western blot to study the effect of parkin deletion and acute knockdown on acetaminophen-induced mitophagy and liver injury in mice
|Williams JA,Ni HM,Haynes A,Manley S,Li Y,Jaeschke H,Ding WX||The Journal of biological chemistry (290:10934)||2015|
|Not Applicable||Not Cited||
Progesterone receptor activation downregulates GATA3 by transcriptional repression and increased protein turnover promoting breast tumor growth.
PA5-16777 was used in western blot to determine the role of progesterone receptor activation in increasing protein turnover and downregulation of GATA3 transcriptional repression which promotes breast tumor growth
|Izzo F,Mercogliano F,Venturutti L,Tkach M,Inurrigarro G,Schillaci R,Cerchietti L,Elizalde PV,Proietti CJ||Breast cancer research : BCR (16:null)||2014|
Synergistic signaling of KRAS and thyroid hormone receptor ß mutants promotes undifferentiated thyroid cancer through MYC up-regulation.
PA5-16777 was used in western blot to examine undifferentiated thyroid cancer in mice
|Zhu X,Zhao L,Park JW,Willingham MC,Cheng SY||Neoplasia (New York, N.Y.) (16:757)||2014|
Antitumor effect of metformin in esophageal cancer: in vitro study.
PA5-16777 was used in western blot to study the molecular mechanisms underlying the anti-proliferative effects of metformin in esophageal cancer
|Kobayashi M,Kato K,Iwama H,Fujihara S,Nishiyama N,Mimura S,Toyota Y,Nomura T,Nomura K,Tani J,Miyoshi H,Kobara H,Mori H,Murao K,Masaki T||International journal of oncology (42:517)||2013|
Smoothened controls cyclin D2 expression and regulates the generation of intermediate progenitors in the developing cortex.
PA5-16777 was used in immunohistochemistry to study the modulation of cyclin D2 expression by Smoothened and its role in the generation of neocortical intermediate progenitors
|Komada M,Iguchi T,Takeda T,Ishibashi M,Sato M||Neuroscience letters (547:87)||2013|
Epithelial progesterone receptor exhibits pleiotropic roles in uterine development and function.
PA5-16777 was used in immunohistochemistry to study the roles of the epithelial progesterone receptor in the development and function of the murine uterus
|Franco HL,Rubel CA,Large MJ,Wetendorf M,Fernandez-Valdivia R,Jeong JW,Spencer TE,Behringer RR,Lydon JP,Demayo FJ||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (26:1218)||2012|