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Immunofluorescence analysis of Cyclin D2 was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclin D2 (DCS-3.1 + DCS-5.2) Mouse Monoclonal Antibody (MA512731) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Avian, Mouse, Human|
|Host / Isotype||Mouse / IgG2a/IgG2b|
|Clone||DCS-3.1 + DCS-5.2|
|Immunogen||Purified histidine-tagged human recombinant full length cyclin D2 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||1-2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12731 targets Cyclin D2 in FACS, IF, and WB applications and shows reactivity with Human, mouse, and Rat samples.
The MA5-12731 immunogen is purified histidine-tagged human recombinant full length cyclin D2 protein.
Cyclin D2 is a G1 cyclin required for G1-phase progression and is a strong candidate for a proto-oncogene. cyclin D2 can phosphorylate pRB when associated with cdk4 and/or cdk6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Smoothened controls cyclin D2 expression and regulates the generation of intermediate progenitors in the developing cortex.
MA5-12731 was used in immunohistochemistry to study the modulation of cyclin D2 expression by Smoothened and its role in the generation of neocortical intermediate progenitors
|Komada M,Iguchi T,Takeda T,Ishibashi M,Sato M||Neuroscience letters (547:87)||2013|
Selective cortical interneuron and GABA deficits in cyclin D2-null mice.
MA5-12731 was used in immunohistochemistry to study selective cortical interneuron and GABA deficits in cyclin D2-null mice
|Glickstein SB,Moore H,Slowinska B,Racchumi J,Suh M,Chuhma N,Ross ME||Development (Cambridge, England) (134:4083)||2007|
Loss of cyclin D1 impairs cerebellar development and suppresses medulloblastoma formation.
MA5-12731 was used in immunohistochemistry to study the role of cyclin D1 in cerebellar development and medulloblastoma formation
|Pogoriler J,Millen K,Utset M,Du W||Development (Cambridge, England) (133:3929)||2006|
Cyclin D2 compensates for the loss of cyclin D1 in estrogen-induced mouse uterine epithelial cell proliferation.
MA5-12731 was used in immunohistochemistry and western blot to study the ability of cyclin D2 to compensate for cyclin D1 loss in estrogen-induced mouse uterine epithelial cell proliferation
|Chen B,Pollard JW||Molecular endocrinology (Baltimore, Md.) (17:1368)||2003|
Sustained expression of the transcription factor GLIS3 is required for normal beta cell function in adults.
MA5-12731 was used in western blot to study the role of the GLIS3 transcription factor in normal pancreatic beta-cell maintenance and proliferation in response to a high fat diet in adult mice
|Yang Y,Chang BH,Chan L||EMBO molecular medicine (5:92)||2013|
GSK-3 inactivation or depletion promotes β-cell replication via down regulation of the CDK inhibitor, p27 (Kip1).
MA5-12731 was used in western blot to study the mechanism by which GSK-3 inactivation or depletion promotes beta-cell replication
|Stein J,Milewski WM,Hara M,Steiner DF,Dey A||Islets (3:21)||2011|
Adaptive beta-cell proliferation is severely restricted with advanced age.
MA5-12731 was used in western blot to study the impact of ageing on adaptive pancreatic beta-cell proliferation
|Rankin MM,Kushner JA||Diabetes (58:1365)||2009|
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
MA5-12731 was used in western blot to study the role of CDK4 in cell cycle regulation using targeted knockdown
|Tsutsui T,Hesabi B,Moons DS,Pandolfi PP,Hansel KS,Koff A,Kiyokawa H||Molecular and cellular biology (19:7011)||1999|
Regulation of exit from quiescence by p27 and cyclin D1-CDK4.
MA5-12731 was used in western blot to study the role of p27 and cyclin D1-CDK4 in regulating S-phase entry of quiescent NIH 3T3 cells
|Ladha MH,Lee KY,Upton TM,Reed MF,Ewen ME||Molecular and cellular biology (18:6605)||1998|
Cyclin D3 compensates for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression.
MA5-12731 was used in immunoprecipitation and western blot to study the ability of cyclin D3 to compensate for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression
|Zhang Q,Sakamoto K,Liu C,Triplett AA,Lin WC,Rui H,Wagner KU||Cancer research (71:7513)||2011|
v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity.
MA5-12731 was used in immunoprecipitation to study the mechanism by which v-Jun promotes S-phase entry in the absence of mitogens
|Clark W,Black EJ,MacLaren A,Kruse U,LaThangue N,Vogt PK,Gillespie DA||Molecular and cellular biology (20:2529)||2000|
G1/S-specific cyclin D2; G1/S-specific cyclin-D2; vin-1 proto-oncogene
2600016F06Rik; AI256817; BF642806; C86853; CCND2; cD2; Cyl-2; KIAK0002; MPPH3; Vin-1; Vin1