|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Avian, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||His-tagged recombinant full-length Cyclin D2|
|Contains||0.08% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Cyclin D2 is a G1 cyclin required for G1 phase progression and is a strong candidate for a proto-oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk 4 and / or cdk 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Sustained expression of the transcription factor GLIS3 is required for normal beta cell function in adults.
MA1-12297 was used in western blot to study the role of the GLIS3 transcription factor in normal pancreatic beta-cell maintenance and proliferation in response to a high fat diet in adult mice
|Yang Y,Chang BH,Chan L||EMBO molecular medicine (5:92)||2013|
GSK-3 inactivation or depletion promotes ß-cell replication via down regulation of the CDK inhibitor, p27 (Kip1).
MA1-12297 was used in western blot to study the mechanism by which GSK-3 inactivation or depletion promotes beta-cell replication
|Stein J,Milewski WM,Hara M,Steiner DF,Dey A||Islets (3:21)||2011|
Control of cyclin D1 and breast tumorigenesis by the EglN2 prolyl hydroxylase.
MA1-12297 was used in western blot to study the role of EglN2 prolyl hydroxylase in breast carcinogenesis
|Zhang Q,Gu J,Li L,Liu J,Luo B,Cheung HW,Boehm JS,Ni M,Geisen C,Root DE,Polyak K,Brown M,Richardson AL,Hahn WC,Kaelin WG,Bommi-Reddy A||Cancer cell (16:413)||2009|
Adaptive beta-cell proliferation is severely restricted with advanced age.
MA1-12297 was used in western blot to study the impact of ageing on adaptive pancreatic beta-cell proliferation
|Rankin MM,Kushner JA||Diabetes (58:1365)||2009|
Functions of p21 and p27 in the regenerating epithelial linings of the mouse small and large intestine.
MA1-12297 was used in western blot to investigate the role of p21 and p27 in intestinal epithelial regeneraton
|Zheng Y,Bie W,Yang R,Perekatt AO,Poole AJ,Tyner AL||Cancer biology and therapy (7:873)||2008|
Molecular characterization of spontaneous mesenchymal stem cell transformation.
MA1-12297 was used in western blot to investigate the molecular mechanisms of spontaneous mesenchymal stem cell transformation
|Rubio D,Garcia S,Paz MF,De la Cueva T,Lopez-Fernandez LA,Lloyd AC,Garcia-Castro J,Bernad A||PloS one (3:null)||2008|
Effects of granulosa cell-specific deletion of Rb in Inha-alpha null female mice.
MA1-12297 was used in western blot to investigate the role of RB protein in ovarian tumorigenesis
|Andreu-Vieyra C,Chen R,Matzuk MM||Endocrinology (148:3837)||2007|
|Not Applicable||Not Cited||
p75NTR enhances PC12 cell tumor growth by a non-receptor mechanism involving downregulation of cyclin D2.
MA1-12297 was used in western blot to elucidate how p75NTR suppresses cyclin D2 expression in PC12 cells
|Fritz MD,Mirnics ZK,Nylander KD,Schor NF||Experimental cell research (312:3287)||2006|
Pomegranate fruit juice for chemoprevention and chemotherapy of prostate cancer.
MA1-12297 was used in western blot to investigate the protective effect of pomegranate fruit juice against prostate cancer
|Malik A,Afaq F,Sarfaraz S,Adhami VM,Syed DN,Mukhtar H||Proceedings of the National Academy of Sciences of the United States of America (102:14813)||2005|
Involvement of nuclear factor-kappa B, Bax and Bcl-2 in induction of cell cycle arrest and apoptosis by apigenin in human prostate carcinoma cells.
MA1-12297 was used in western blot to investigate apigenin-induced apoptosis in human prostate cancer cells
|Gupta S,Afaq F,Mukhtar H||Oncogene (21:3727)||2002|
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
MA1-12297 was used in western blot to study the role of CDK4 in cell cycle regulation using targeted knockdown
|Tsutsui T,Hesabi B,Moons DS,Pandolfi PP,Hansel KS,Koff A,Kiyokawa H||Molecular and cellular biology (19:7011)||1999|
Regulation of exit from quiescence by p27 and cyclin D1-CDK4.
MA1-12297 was used in western blot to study the role of p27 and cyclin D1-CDK4 in regulating S-phase entry of quiescent NIH 3T3 cells
|Ladha MH,Lee KY,Upton TM,Reed MF,Ewen ME||Molecular and cellular biology (18:6605)||1998|
Cyclin D3 compensates for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression.
MA1-12297 was used in immunoprecipitation and western blot to study the ability of cyclin D3 to compensate for the loss of cyclin D1 during ErbB2-induced mammary tumor initiation and progression
|Zhang Q,Sakamoto K,Liu C,Triplett AA,Lin WC,Rui H,Wagner KU||Cancer research (71:7513)||2011|
v-Jun overrides the mitogen dependence of S-phase entry by deregulating retinoblastoma protein phosphorylation and E2F-pocket protein interactions as a consequence of enhanced cyclin E-cdk2 catalytic activity.
MA1-12297 was used in immunoprecipitation to study the mechanism by which v-Jun promotes S-phase entry in the absence of mitogens
|Clark W,Black EJ,MacLaren A,Kruse U,LaThangue N,Vogt PK,Gillespie DA||Molecular and cellular biology (20:2529)||2000|
Cyclin D2 protein stability is regulated in pancreatic beta-cells.
MA1-12297 was used in immunohistochemistry and western blot to study the regulation of cyclin D2 protein stability in pancreatic beta-cells
|He LM,Sartori DJ,Teta M,Opare-Addo LM,Rankin MM,Long SY,Diehl JA,Kushner JA||Molecular endocrinology (Baltimore, Md.) (23:1865)||2009|
Selective cortical interneuron and GABA deficits in cyclin D2-null mice.
MA1-12297 was used in immunohistochemistry to study selective cortical interneuron and GABA deficits in cyclin D2-null mice
|Glickstein SB,Moore H,Slowinska B,Racchumi J,Suh M,Chuhma N,Ross ME||Development (Cambridge, England) (134:4083)||2007|
Loss of cyclin D1 impairs cerebellar development and suppresses medulloblastoma formation.
MA1-12297 was used in immunohistochemistry to study the role of cyclin D1 in cerebellar development and medulloblastoma formation
|Pogoriler J,Millen K,Utset M,Du W||Development (Cambridge, England) (133:3929)||2006|
Cyclin D2 compensates for the loss of cyclin D1 in estrogen-induced mouse uterine epithelial cell proliferation.
MA1-12297 was used in immunohistochemistry and western blot to study the ability of cyclin D2 to compensate for cyclin D1 loss in estrogen-induced mouse uterine epithelial cell proliferation
|Chen B,Pollard JW||Molecular endocrinology (Baltimore, Md.) (17:1368)||2003|