|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Dog, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified human recombinant full length cyclin D3 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12717 targets Cyclin D3 in FACS, IF, IHC (P), IP, and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-12717 immunogen is purified human recombinant full length cyclin D3 protein.
Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. Cyclins D1, D2, and D3 form active kinase complexes in vivo with a distinct subset of cdks, particularly with cdk4 and cdk6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of mutant BRAF splice variant signaling by next-generation, selective RAF inhibitors.
MA5-12717 was used in western blot to study the ability of next-generation RAF inhibitors to inhibit signaling mediated by splice variants of mutant BRAF
|Basile KJ,Le K,Hartsough EJ,Aplin AE||Pigment cell and melanoma research (27:479)||2014|
CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point.
MA5-12717 was used in western blot to study the roles CDK7 and p21 in regulating the T172 phosphorylation of CDK4 at the restriction point
|Bisteau X,Paternot S,Colleoni B,Ecker K,Coulonval K,De Groote P,Declercq W,Hengst L,Roger PP||PLoS genetics (9:null)||2013|
Differential regulation of cyclin-dependent kinase 4 (CDK4) and CDK6, evidence that CDK4 might not be activated by CDK7, and design of a CDK6 activating mutation.
MA5-12717 was used in western blot to study differences in the activation of the cyclin-dependent kinases Cdk4 and Cdk6 in intact cells
|Bockstaele L,Bisteau X,Paternot S,Roger PP||Molecular and cellular biology (29:4188)||2009|
|Not Applicable||Not Cited||
Identification of a potent natural triterpenoid inhibitor of proteosome chymotrypsin-like activity and NF-kappaB with antimyeloma activity in vitro and in vivo.
MA5-12717 was used in western blot to screen for compounds that suppress cyclin D2 promoter transcription
|Tiedemann RE,Schmidt J,Keats JJ,Shi CX,Zhu YX,Palmer SE,Mao X,Schimmer AD,Stewart AK||Blood (113:4027)||2009|
Orphan receptor small heterodimer partner suppresses tumorigenesis by modulating cyclin D1 expression and cellular proliferation.
MA5-12717 was used in western blot to study the mechanism by which the nuclear receptor family member SHP suppresses hepatic carcinogenesis
|Zhang Y,Xu P,Park K,Choi Y,Moore DD,Wang L||Hepatology (Baltimore, Md.) (48:289)||2008|
Nuclear localization of barrier-to-autointegration factor is correlated with progression of S phase in human cells.
MA5-12717 was used in western blot to investigate the correlation between nuclear localization of barrier-to-autointegration factor and S phase progression in human cells
|Haraguchi T,Koujin T,Osakada H,Kojidani T,Mori C,Masuda H,Hiraoka Y||Journal of cell science (120:1967)||2007|
Differential utilization of cyclin D1 and cyclin D3 in the distinct mitogenic stimulations by growth factors and TSH of human thyrocytes in primary culture.
MA5-12717 was used in western blot to study the roles of cyclin D1 and cyclin D3 in different mitogenic modes of human thyrocytes
|Paternot S,Dumont JE,Roger PP||Molecular endocrinology (Baltimore, Md.) (20:3279)||2006|
Cyclin D1 is transcriptionally regulated by and required for transformation by activated signal transducer and activator of transcription 3.
MA5-12717 was used in western blot to study the role of STAT3 in regulating cyclin D1 expression and the correlation between elevated cyclin D1 and activated STAT3 in breast cancer
|Leslie K,Lang C,Devgan G,Azare J,Berishaj M,Gerald W,Kim YB,Paz K,Darnell JE,Albanese C,Sakamaki T,Pestell R,Bromberg J||Cancer research (66:2544)||2006|
Differential involvement of the actin cytoskeleton in differentiation and mitogenesis of thyroid cells: inactivation of Rho proteins contributes to cyclic adenosine monophosphate-dependent gene expression but prevents mitogenesis.
MA5-12717 was used in western blot to study the role of Rho proteins in actin cytoskeleton functions during thyroid cell differentiation and mitogenesis
|Fortemaison N,Blancquaert S,Dumont JE,Maenhaut C,Aktories K,Roger PP,Dremier S||Endocrinology (146:5485)||2005|
Increase in Ara-C cytotoxicity in the presence of valproate, a histone deacetylase inhibitor, is associated with the concurrent expression of cyclin D1 and p27(Kip 1) in acute myeloblastic leukemia cells.
MA5-12717 was used in western blot to investigate the effect of valproate on Ara-C cytotoxicity in acute myeloblastic leukemia cells and its mechanism
|Siitonen T,Koistinen P,Savolainen ER||Leukemia research (29:1335)||2005|
Expression of D-type cyclins in colon cancer and in cell lines from colon carcinomas.
MA5-12717 was used in western blot to study the expression of cyclins D1, D2 and D3 in colon cancer and their role in carcinogenesis
|Mermelshtein A,Gerson A,Walfisch S,Delgado B,Shechter-Maor G,Delgado J,Fich A,Gheber L||British journal of cancer (93:338)||2005|
Cdk6-cyclin D3 activity in murine ES cells is resistant to inhibition by p16(INK4a).
MA5-12717 was used in western blot to study the resistance of embryonic stem cell Cdk6-cyclin D3 complexes to inhibition by p16(INK4a)
|Faast R,White J,Cartwright P,Crocker L,Sarcevic B,Dalton S||Oncogene (23:491)||2004|
Regulation of exit from quiescence by p27 and cyclin D1-CDK4.
MA5-12717 was used in western blot to study the role of p27 and cyclin D1-CDK4 in regulating S-phase entry of quiescent NIH 3T3 cells
|Ladha MH,Lee KY,Upton TM,Reed MF,Ewen ME||Molecular and cellular biology (18:6605)||1998|
Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas.
MA5-12717 was used in immunohistochemistry to study the role of the G1/S transition regulators in the recurrence of head and neck squamous carcinomas
|Canzonieri V,Barzan L,Franchin G,Vaccher E,Talamini R,Sulfaro S,Baldassarre G||Journal of cellular physiology (227:233)||2012|
ß-catenin/cyclin D1 mediated development of suture mesenchyme in calvarial morphogenesis.
MA5-12717 was used in immunohistochemistry to investigate the role of beta-catenin/cyclin D1 during calvarial morphogenesis
|Mirando AJ,Maruyama T,Fu J,Yu HM,Hsu W||BMC developmental biology (10:null)||2010|
Expression of cyclin D2 is an independent predictor of the development of hepatic metastasis in colorectal cancer.
MA5-12717 was used in immunohistochemistry to investigate the prognostic significance cyclin D2 expression in liver metastasis
|Sarkar R,Hunter IA,Rajaganeshan R,Perry SL,Guillou P,Jayne DG||Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland (12:316)||2010|
Cell cycle regulators and outcome of adjuvant cisplatin-based chemotherapy in completely resected non-small-cell lung cancer: the International Adjuvant Lung Cancer Trial Biologic Program.
MA5-12717 was used in immunohistochemistry to study the predictive/prognostic value of cell cycle regulators in adjunct cisplatin chemotherapy of non-small cell lung cancer
|Filipits M,Pirker R,Dunant A,Lantuejoul S,Schmid K,Huynh A,Haddad V,André F,Stahel R,Pignon JP,Soria JC,Popper HH,Le Chevalier T,Brambilla E||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (25:2735)||2007|
Cyclin D1-negative mantle cell lymphoma: a clinicopathologic study based on gene expression profiling.
MA5-12717 was used in immunohistochemistry to study the clinicopathology and gene expression profiles of 6 cases of cyclin D1-negative mantle cell lymphoma
|Fu K,Weisenburger DD,Greiner TC,Dave S,Wright G,Rosenwald A,Chiorazzi M,Iqbal J,Gesk S,Siebert R,De Jong D,Jaffe ES,Wilson WH,Delabie J,Ott G,Dave BJ,Sanger WG,Smith LM,Rimsza L,Braziel RM,Müller-Hermelink HK,Campo E,Gascoyne RD,Staudt LM,Chan WC||Blood (106:4315)||2005|
Nuclear beta-catenin expression is rare and its potential association with short survival in colorectal signet-ring cell carcinoma.
MA5-12717 was used in immunohistochemistry to study the association of nuclear beta-catenin with poor survival in colorectal signet-ring cell carcinoma
|Wong SC,Chan AT,Lo ES,Lo YM||Applied immunohistochemistry and molecular morphology : AIMM (13:248)||2005|
Cyclin D3 is a predictive and prognostic factor in diffuse large B-cell lymphoma.
MA5-12717 was used in immunohistochemistry to study the predictive and prognostic value of cyclin D3 in diffuse large B-cell lymphoma
|Filipits M,Jaeger U,Pohl G,Stranzl T,Simonitsch I,Kaider A,Skrabs C,Pirker R||Clinical cancer research : an official journal of the American Association for Cancer Research (8:729)||2002|
Molecular aberrations of the G1-S checkpoint in myxoid and round cell liposarcoma.
MA5-12717 was used in immunohistochemistry to study the molecular biology of the G1-S cell cycle checkpoint in myxoid and round cell liposarcoma
|Dei Tos AP,Piccinin S,Doglioni C,Vukosavljevic T,Mentzel T,Boiocchi M,Fletcher CD||The American journal of pathology (151:1531)||1997|
In vivo interference with Skp1 function leads to genetic instability and neoplastic transformation.
MA5-12717 was used in immunoprecipitation to study the effect of loss of Skp1 function on genetic instability and neoplastic transformation in a transgenic mouse model
|Piva R,Liu J,Chiarle R,Podda A,Pagano M,Inghirami G||Molecular and cellular biology (22:8375)||2002|
Genetic evidence for the interactions of cyclin D1 and p27(Kip1) in mice.
MA5-12717 was used in immunoprecipitation to study the interactions of cyclin D1 and p27(Kip1) using knockout mice
|Tong W,Pollard JW||Molecular and cellular biology (21:1319)||2001|
Expression of cyclin-dependent kinase inhibitor p27(Kip1) in AIDS-related diffuse large-cell lymphomas is associated with Epstein-Barr virus-encoded latent membrane protein 1.
MA5-12717 was used in immunohistochemistry (paraffin) to examine the role of cell-cycle regulators in the pathogenesis of acquired immune deficiency syndrome-related non-Hodgkin's lymphomas.
|Gloghini A,Gaidano G,Larocca LM,Pierconti F,Cingolani A,Dal Maso L,Capello D,Franceschi S,Tirelli U,Libra M,Niu H,Dalla-Favera R,Carbone A||The American journal of pathology (161:163)||2002|