|Tested species reactivity||Human, Rat|
|Published species reactivity||Dog, Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified human recombinant full length cyclin D3 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12723 targets Cyclin D3 in WB applications and shows reactivity with Human and Rat samples.
The MA5-12723 immunogen is purified human recombinant full length cyclin D3 protein.
Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. D3 can phosphorylate pRB when associated with cdk4 and/or cdk6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
SSeCKS sequesters cyclin D1 in glomerular parietal epithelial cells and influences proliferative injury in the glomerulus.
MA5-12723 was used in western blot to study the cytoplasmic sequestration of cyclin D1 by SSeCKS in glomerular parietal epithelial cells and the significance for glomerular proliferative injury
|Burnworth B,Pippin J,Karna P,Akakura S,Krofft R,Zhang G,Hudkins K,Alpers CE,Smith K,Shankland SJ,Gelman IH,Nelson PJ||Laboratory investigation; a journal of technical methods and pathology (92:499)||2012|
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
MA5-12723 was used in western blot to study the role of CDK4 in cell cycle regulation using targeted knockdown
|Tsutsui T,Hesabi B,Moons DS,Pandolfi PP,Hansel KS,Koff A,Kiyokawa H||Molecular and cellular biology (19:7011)||1999|
Differential utilization of cyclin D1 and cyclin D3 in the distinct mitogenic stimulations by growth factors and TSH of human thyrocytes in primary culture.
MA5-12723 was used in immunoprecipitation to study the roles of cyclin D1 and cyclin D3 in different mitogenic modes of human thyrocytes
|Paternot S,Dumont JE,Roger PP||Molecular endocrinology (Baltimore, Md.) (20:3279)||2006|
Differential involvement of the actin cytoskeleton in differentiation and mitogenesis of thyroid cells: inactivation of Rho proteins contributes to cyclic adenosine monophosphate-dependent gene expression but prevents mitogenesis.
MA5-12723 was used in immunoprecipitation to study the role of Rho proteins in actin cytoskeleton functions during thyroid cell differentiation and mitogenesis
|Fortemaison N,Blancquaert S,Dumont JE,Maenhaut C,Aktories K,Roger PP,Dremier S||Endocrinology (146:5485)||2005|