Immunohistochemistry analysis of Cyclin D3 showing staining in the nucleus of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclin D3 Antibody Mouse Monoclonal Antibody (MA512726) diluted in 3% BSA-PBS at a dilution of 1:50 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Dog, Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified human recombinant full length cyclin D3 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12726 targets Cyclin D3 in WB applications and shows reactivity with Human and Rat samples.
The MA5-12726 immunogen is purified human recombinant full length cyclin D3 protein.
Cyclin D3 is a G1 cyclin closely related to cyclin D1 and D2. D3 can phosphorylate pRB when associated with cdk4 and/or cdk6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CDK4 T172 phosphorylation is central in a CDK7-dependent bidirectional CDK4/CDK2 interplay mediated by p21 phosphorylation at the restriction point.
MA5-12726 was used in immunoprecipitation to study the roles CDK7 and p21 in regulating the T172 phosphorylation of CDK4 at the restriction point
|Bisteau X,Paternot S,Colleoni B,Ecker K,Coulonval K,De Groote P,Declercq W,Hengst L,Roger PP||PLoS genetics (9:null)||2013|
Differential utilization of cyclin D1 and cyclin D3 in the distinct mitogenic stimulations by growth factors and TSH of human thyrocytes in primary culture.
MA5-12726 was used in immunoprecipitation to study the roles of cyclin D1 and cyclin D3 in different mitogenic modes of human thyrocytes
|Paternot S,Dumont JE,Roger PP||Molecular endocrinology (Baltimore, Md.) (20:3279)||2006|
Differential involvement of the actin cytoskeleton in differentiation and mitogenesis of thyroid cells: inactivation of Rho proteins contributes to cyclic adenosine monophosphate-dependent gene expression but prevents mitogenesis.
MA5-12726 was used in immunoprecipitation to study the role of Rho proteins in actin cytoskeleton functions during thyroid cell differentiation and mitogenesis
|Fortemaison N,Blancquaert S,Dumont JE,Maenhaut C,Aktories K,Roger PP,Dremier S||Endocrinology (146:5485)||2005|
Combined effect of cyclin D3 expression and abrogation of cyclin D1 prevent mouse skin tumor development.
MA5-12726 was used in western blot to study the protective effect against skin cancer formation of cyclin D3 overexpression plus cyclin D1 ablation
|Wang X,Sistrunk C,Miliani de Marval PL,Kim Y,Rodriguez-Puebla ML||Cell cycle (Georgetown, Tex.) (11:335)||2012|
Targeted disruption of CDK4 delays cell cycle entry with enhanced p27(Kip1) activity.
MA5-12726 was used in western blot to study the role of CDK4 in cell cycle regulation using targeted knockdown
|Tsutsui T,Hesabi B,Moons DS,Pandolfi PP,Hansel KS,Koff A,Kiyokawa H||Molecular and cellular biology (19:7011)||1999|