Immunohistochemistry analysis of Cyclophilin B showing staining in the cytoplasm and weak staining in the nucleus of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclophilin B Mouse Monoclonal Antibody (37-0600) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein encompassing the C-terminal region of human Cyclophilin B|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cyclophilin B as a co-regulator of prolactin-induced gene expression and function in breast cancer cells.
37-0600 was used in western blot to assess the effect of cyclophilin B knockdown on prolactin signaling in breast cancer cells
|Fang F,Zheng J,Galbaugh TL,Fiorillo AA,Hjort EE,Zeng X,Clevenger CV||Journal of molecular endocrinology (44:319)||2010|
|Human||Not Cited||Expression of cyclophilin B is associated with malignant progression and regulation of genes implicated in the pathogenesis of breast cancer.||Fang F,Flegler AJ,Du P,Lin S,Clevenger CV||The American journal of pathology (174:297)||2009|
|Not Applicable||Not Cited||
The isolation of novel mesenchymal stromal cell chemotactic factors from the conditioned medium of tumor cells.
37-0600 was used in immunocytochemistry to use mass spectroscopy to identify factors that regulate mesenchymal stromal cell chemotaxis
|Lin SY,Yang J,Everett AD,Clevenger CV,Koneru M,Mishra PJ,Kamen B,Banerjee D,Glod J||Experimental cell research (314:3107)||2008|