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|Tested species reactivity||Human, Rat|
|Published species reactivity||Rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues G(57) I G H T T G K P L H F K G C(71) of human Cyp-40.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA3-024 detects cyclophilin 40 (CyP-40) from human and rat tissues and cells.
PA3-024 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 40 kDa protein representing CyP-40 from rat brain extract.
The PA3-024 immunogen is a synthetic peptide corresponding to residues G(57) I G H T T G K P L H F K G C(71) of human Cyp-40.
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents: cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP).
Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.
CyP-40, a 40 kDa protein, shares significant homology with smaller CyPA (CyP-18) and FKBP59. CyP-40 exhibits the characteristic CsA binding and isomerase activity of CyP-18, though these activities appear to be less with CyP-40 than with Cyp-18. Like FKBP59, CyP-40 has been found in progesterone receptor complexes. CyP-40 is expressed at similar levels in many tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The 23-kDa acidic protein in reticulocyte lysate is the weakly bound component of the hsp foldosome that is required for assembly of the glucocorticoid receptor into a functional heterocomplex with hsp90.
PA3-024 was used in western blot to study the function of p23 during the process of receptor activation by hsp foldosome
|Hutchison KA,Stancato LF,Owens-Grillo JK,Johnson JL,Krishna P,Toft DO,Pratt WB||The Journal of biological chemistry (270:18841)||1995|
40 kDa peptidyl-prolyl cis-trans isomerase; 40 kDa peptidyl-prolyl cis-trans isomerase D; cyclophilin 40; Cyclophilin D; cyclophilin-40; cyclophilin-related protein; Cyp 40; CypD; peptidyl-prolyl cis-trans isomerase D; PPIase D; PPID; rotamase D; testicular tissue protein Li 147
CYP-40; CYP40; CYPD; PPID